Molecular imaging

• US 6,613,210 B1
• Filed: 01/21/2000
• Issued: 09/02/2003
• Est. Priority Date: 05/10/1995
• Status: Expired due to Term

• Current Assignee: deltaDOT Limited
• Original Assignee: Imperial College of Science Technology & Medicine (Imperial College London)
• Inventors: Hassard, Stuart, Mainwood, Alison Mary, Hassard, John Francis
• Primary Examiner(s): Nguyen, Nam
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/489,109
• Time in Patent Office: 1,320 Days
• Field of Search: 204/450, 204/452, 204/461, 204/600, 204/612, 204/603
• US Class Current: 204/461
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

A method of imaging molecules of interest within a biological sample includes shining a UV light onto the sample and detecting the molecular UV absorption. Where the molecules of interest are themselves UV absorbers, the intrinsic absorption of those molecules may be used. If the molecules of interest are not good UV absorbers, UV-aborbing tag molecules may be use. The method may be used in molecular imaging devices of all types, and in DNA sequences. A novel diamond-based detector is disclosed which is suitable for many applications.

Claims

1. A method of analyzing a protein sample comprising the steps of:

(a) loading the protein sample onto an electrophoresis material;

(b) applying a potential difference along the material, thereby causing the sample to move along the material;

(c) shining ultra-violet light onto the sample; and

(d) repeatedly measuring the intrinsic UV absorption of the sample at a plurality of different detector positions on the electrophoresis material to directly digitally image the different proteins present in the sample, quantify the amounts of the different proteins and separate them by molecular weight.

2. The method of claim 1 wherein the proteins are imaged in the time domain, the proteins being detected at spatially-fixed positions as they move on or within the electrophoresis material.

3. The method of claim 1 wherein the step of digitally imaging the proteins is carried out by a detector of UV-enhanced silicon.

4. The method of claim 1 wherein the step of digitally imaging the protein is carried out by a detector of diamond.

5. The method of claim 1 further including the step of analysing the imaged proteins to ascertain the effect of nutrients, environmental factors, disease or drugs on a human or animal patient from which the sample was derived.

6. The method of claim 1 in which the protein sample is derived from blood serum.

7. The method of claim 1, further including the step of switching the ultra violet wavelength using a wavelength-switchable lamp.

8. The method of claim 1, wherein the wavelength is switchable between a value less than 230 nm to a value of 280 nm.

9. A method of analyzing a protein sample comprising the steps of:

(a) loading the protein sample onto an electrophoresis material;

(b) applying a potential difference along the material, thereby causing the sample to move along the material;

(c) shining ultra-violet light onto the sample; and

(d) repeatedly measuring the intrinsic UV absorption of the sample at a plurality of different detector positions on the electrophoresis material to directly digitally image the different proteins present in the sample in two dimensions, quantify the amounts of the different proteins and separate them by molecular weight.

10. A method of measuring the effects of nutrients, environmental factors, disease or drugs on a human or animal patient comprising the steps of:

(a) obtaining a protein sample from the patient and loading the sample onto an electrophoresis material;

(b) applying a potential difference along the material, thereby causing the sample to move along the material;

(c) shining ultra-violet light onto the sample;

(d) repeatedly measuring the intrinsic UV absorption of the sample at a plurality of different detector positions on the electrophoresis material to directly digitally image the different proteins present in the sample, quantify the amounts of the different proteins and separate them by molecular weight; and

(e) measuring the said effects by analyzing the imaged proteins.

11. The method of claim 10, further including the step of switching the ultra violet wavelength using a wavelength-switchable lamp.

12. The method of claim 11, wherein the wavelength is switchable between a value less than 230 nm to a value of 280 nm.