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Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques

  • US 6,613,508 B1
  • Filed: 07/22/1997
  • Issued: 09/02/2003
  • Est. Priority Date: 01/23/1996
  • Status: Expired due to Term
First Claim
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1. A method for determining the identity of a nucleic acid molecule, comprising sequentially:

  • (a) generating tagged nucleic acid molecules from one or more selected target nucleic acid molecules, wherein a tag is correlative with a particular nucleic acid fragment and detectable by non-fluorescent spectrometry or potentiometry;

    (b) separating said tagged molecules by size;

    (c) cleaving said tag from said tagged molecule; and

    (d) detecting said tag by non-fluorescent spectrometry or potentiometry, and therefrom determining the identity of said nucleic acid molecule.

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