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Apparatus and method for correcting for variable velocity in microfluidic systems

  • US 6,613,512 B1
  • Filed: 12/05/2000
  • Issued: 09/02/2003
  • Est. Priority Date: 06/09/1997
  • Status: Expired due to Term
First Claim
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1. A method for determining the rate or extent of a reaction or assay in a microfluidic system, comprising:

  • converting a first reaction or assay component having a first velocity (U1) into an assay product having a second velocity (Up) in a microfluidic channel;

    determining at least one velocity selected from the group consisting of U1 and Up;

    determining the concentration of the first reaction component [C1] or the concentration of the assay product [Cp] in a portion of the microfluidic channel; and

    determining the rate or extent of the reaction or assay, wherein (U1)[C1]converted=(Up)[Cp] or (U1)[C1]=(Up)[Cp].

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