Methods for the survey and genetic analysis of populations
First Claim
1. A method to determine the genetic diversity of a population by determining the DNA sequence of a targeted polymorphic region from one gene that is common to all, or the majority of all, members of the population, comprising the steps of:
- a) providing a sample containing a population that comprises at least one genomic DNA molecule containing regions of high sequence conservation flanking a region of polymorphic sequence;
b) isolating the genomic DNA molecule from the sample;
c) performing PCR on the genomic DNA molecule, to amplify a subregion of the region of polymorphic sequence, using an upstream primer comprising a binding site for a solid support and a downstream primer to produce an amplified fragment, wherein the upstream primer and the downstream primer hybridize to the regions of high sequence conservation flanking the region of polymorphic sequence;
d) cleaving the amplified fragment with a restriction enzyme that cleaves the amplified fragment at a site adjacent to the region of polymorphic sequence;
e) binding the cleaved amplified fragment to a solid support to form an immobilized product;
f) splitting the immobilized product into two pools;
g) attaching a double-stranded linker to the immobilized product of each pool, wherein the linker comprises a Type IIS restriction enzyme site;
h) digesting the immobilized product with a Type IIS restriction enzyme to form a tag;
i) ligating the tag to a second tag to form a ditag;
j) amplifying the ditag by PCR;
k) cleaving the ditag with the restriction enzyme of step d) and ligating the ditag to a second ditag to form a concatemer; and
l) determining a DNA sequence of the concatemer, wherein the sequence of the concatemer is a sequence of a multitude of markers, which provides a measure of the genetic diversity of a population, thereby allowing for the determination of the genetic diversity of a population.
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Abstract
The present invention relates to methods for performing surveys of the genetic diversity of a population. The invention also relates to methods for performing genetic analyses of a population. The invention further relates to methods for the creation of databases comprising the survey information and the databases created by these methods. The invention also relates to methods for analyzing the information to correlate the presence of nucleic acid markers with desired parameters in a sample. These methods have application in the fields of geochemical exploration, agriculture, bioremediation, environmental analysis, clinical microbiology, forensic science and medicine.
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Citations
19 Claims
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1. A method to determine the genetic diversity of a population by determining the DNA sequence of a targeted polymorphic region from one gene that is common to all, or the majority of all, members of the population, comprising the steps of:
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a) providing a sample containing a population that comprises at least one genomic DNA molecule containing regions of high sequence conservation flanking a region of polymorphic sequence;
b) isolating the genomic DNA molecule from the sample;
c) performing PCR on the genomic DNA molecule, to amplify a subregion of the region of polymorphic sequence, using an upstream primer comprising a binding site for a solid support and a downstream primer to produce an amplified fragment, wherein the upstream primer and the downstream primer hybridize to the regions of high sequence conservation flanking the region of polymorphic sequence;
d) cleaving the amplified fragment with a restriction enzyme that cleaves the amplified fragment at a site adjacent to the region of polymorphic sequence;
e) binding the cleaved amplified fragment to a solid support to form an immobilized product;
f) splitting the immobilized product into two pools;
g) attaching a double-stranded linker to the immobilized product of each pool, wherein the linker comprises a Type IIS restriction enzyme site;
h) digesting the immobilized product with a Type IIS restriction enzyme to form a tag;
i) ligating the tag to a second tag to form a ditag;
j) amplifying the ditag by PCR;
k) cleaving the ditag with the restriction enzyme of step d) and ligating the ditag to a second ditag to form a concatemer; and
l) determining a DNA sequence of the concatemer, wherein the sequence of the concatemer is a sequence of a multitude of markers, which provides a measure of the genetic diversity of a population, thereby allowing for the determination of the genetic diversity of a population. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification