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PCR microreactor for amplifying DNA using microquantities of sample fluid

  • US 6,613,560 B1
  • Filed: 02/11/2000
  • Issued: 09/02/2003
  • Est. Priority Date: 10/19/1994
  • Status: Expired due to Fees
First Claim
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1. In a device for conducting the polymerase chain reaction (PCR) process comprising a reaction chamber defined by two or more interior surfaces, a means for introducing PCR reaction components into the chamber, a means for removing the PCR reaction product from the chamber, and a means for controlling the temperature of the reaction chamber, the improvement which comprises:

  • fabricating the device from a chemically inert material that does not contain silicon and is thermally, chemically and mechanically stable under the conditions at which a PCR reaction is conducted, and employing a reaction chamber adapted to contain in the range of approximately 1 μ

    l to 500 μ

    l of fluid, wherein the interior surfaces of the reaction chamber are coated with a sorption-reducing polyethylene oxide layer so as to enhance thermal stability, chemical stability and biofouling resistance.

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