Hematopoietic differentiation of human embryonic stem cells

• US 6,613,568 B2
• Filed: 08/27/2001
• Issued: 09/02/2003
• Est. Priority Date: 11/08/1999
• Status: Expired due to Term

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Thomson, James A., Kaufman, Dan S.
• Primary Examiner(s): Crouch, Deborah
• Application Number:
  US09/940,175
• Publication Number:
  US 20020015694A1
• Time in Patent Office: 736 Days
• Field of Search: 424/93.1, 424/93.2, 435/325, 435/366, 435/375
• US Class Current: 435/377
• CPC Class Codes: A61K 2035/122 for inducing tolerance or supression of immune responses
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2502/13 connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
• CPC Class Codes: C12N 2502/1394 Bone marrow stromal cells; whole marrow
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors

Abstract

Disclosed herein are methods of obtaining human hematopoietic cells from human embryonic stem cells using mammalian stromal cells. Hematopoietic cells derived in this way are useful for creating cell cultures suitable for transplantation, transfusion, and other purposes.

Claims

1. A method for obtaining human hematopoietic cells, comprising exposing a human embryonic stem cell culture to mammalian hematopoietic stromal cells so as to thereby produce human hematopoietic cells.