Microfluidic analytic detection assays, devices, and integrated systems
First Claim
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1. A method for detecting at least one component of interest in a mixture of components in a microfluidic system, the method comprising the steps of:
- (i) flowing the mixture of components, which mixture of components comprises the at least one component of interest, through a separation channel region in a first microfluidic channel, resulting in one or more separated components, each of the one or more separated components bearing a first detectable label;
(ii) detecting the first detectable label associated with the one or more separated components in a first detection channel region located downstream of the separation channel region to determine a physical property of the one or more separated components based on the retention time of the one or more separated components through the separation channel region;
(iii) contacting the one or more separated components with a component binding moiety bearing a second detectable label in the first or a second microfluidic channel in a channel region located downstream from the first detection channel region, thereby binding at least a portion of the at least one component of interest to the component-binding moiety;
(iv) detecting at least the second detectable labels in a second detection channel region of the first or the second microfluidic channel to thereby provide a detection signal corresponding to the at least one component of interest; and
(v) deconvoluting the detection signal to identify the component of interest.
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Abstract
Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
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Citations
32 Claims
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1. A method for detecting at least one component of interest in a mixture of components in a microfluidic system, the method comprising the steps of:
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(i) flowing the mixture of components, which mixture of components comprises the at least one component of interest, through a separation channel region in a first microfluidic channel, resulting in one or more separated components, each of the one or more separated components bearing a first detectable label;
(ii) detecting the first detectable label associated with the one or more separated components in a first detection channel region located downstream of the separation channel region to determine a physical property of the one or more separated components based on the retention time of the one or more separated components through the separation channel region;
(iii) contacting the one or more separated components with a component binding moiety bearing a second detectable label in the first or a second microfluidic channel in a channel region located downstream from the first detection channel region, thereby binding at least a portion of the at least one component of interest to the component-binding moiety;
(iv) detecting at least the second detectable labels in a second detection channel region of the first or the second microfluidic channel to thereby provide a detection signal corresponding to the at least one component of interest; and
(v) deconvoluting the detection signal to identify the component of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification