Specific oligonucleotide primers for detection of bovine male chromosome presence by polymerase chain reaction and method
First Claim
Patent Images
1. A polymerase chain reaction primer the nucleotide sequence of which consists of 5′
- -GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID NO;
1).
8 Assignments
0 Petitions
Accused Products
Abstract
This invention is a specific set of oligonucleotide Polymerase chain reaction (PCR) primers [5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1) and 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2)] for specific polymerase chain reaction (PCR) amplification of a region of the bovine male-specific chromosome sequence.
63 Citations
16 Claims
-
1. A polymerase chain reaction primer the nucleotide sequence of which consists of 5′
- -GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID NO;
1).
- -GTGATCCGGCATATAGCTGAGA-3′
-
2. A polymerase chain reaction primer the nucleotide sequence of which consists of 5′
- -TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID NO;
2).
- -TGGTCGCTGATCAGGATGGAA-3′
-
3. A primer pair used for amplifying DNA of the bovine TSPY gene by polymerase chain reaction, wherein the primer pair comprises a first primer comprising the nucleotide sequence of 5′
- -TGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1), and a second primer the nucleotide sequence of 5′
-TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID No.
2). - View Dependent Claims (4)
- -TGATCCGGCATATAGCTGAGA-3′
-
5. A kit useful to detect the presence of a portion of DNA sequence from Y chromosome, comprising:
-
a a first container containing a first oligonucleotide primer comprising the nucleotide sequence 5′
-GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1), wherein said first oligonucleotide primer binds to a specific Y chromosome polynucleotide sequence; and
b at least a second container containing a reagent useful in performance of a polyrnerase chain reaction amplification. - View Dependent Claims (7, 8, 9, 15)
-
-
6. A kit useful to detect the presence of a portion of DNA sequence from Y chromosome, comprising:
-
a a first container containing a second oligonucleotide primer comprising the nucleotide sequence 5′
-TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID No.
2), wherein said second oligonucleotide primer binds to a specific Y chromosome polynucleotide sequence; and
b at least a second container containing a reagent useful in performance of a polymerase chain reaction amplification.
-
-
10. A method of detecting a specific Y chromosome sequence by polymerase chain reaction, comprising the steps of:
-
a amplifying a specific Y chromosome polynucleotide sequence with a primer pair, wherein said primer pair comprises a first primer comprising the nucleotide sequence of 5′
-GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1) and a second primer comprising the nucleotide sequence of 5′
-TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID No.
2); and
b detecting the polynucleotide product of said step of amplifying said specific Y chromosome polynucleotide.
-
-
11. A method of identifying the gender of an animal, comprising the steps of:
-
a obtaining a meat sample from said animal;
b amplifying a target nucleic acid sequence in said meat with a primer comprising the nucleotide sequence of 5′
-GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1);
c isolating amplified products from said genetic material;
d determining the existence of Y chromosome polynucleotide sequence in said meat. - View Dependent Claims (12, 13)
-
-
14. A method of amplifying a specific Y chromosome polynucleotide sequence by polymerase chain reaction, comprising the steps of:
-
a establishing a specific Y chromosome polynucleotide sequence in a solution;
b providing a primer pair, wherein said primer pair comprises a first primer comprising the sequence of 5′
-GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1) and a second primer comprising the sequence of 5′
-TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID No.
2) in said solution;
c denaturing said specific Y chromosome sequence;
d annealing each of said primer pair to a specific region on said specific Y chromosome sequence; and
e extending each of said primer pair on said specific Y chromosome sequence using said specific Y chromosome sequence as a template to amplify a portion of said Y chromosome sequence.
-
-
16. A method of identifying the gender of an animal by polymerase chain reaction, comprising the steps of:
-
a establishing a DNA material from a sample of meat of said animal in a solution;
b providing a primer pair, wherein said primer pair comprises a first primer comprising the sequence of 5′
-GTGATCCGGCATATAGCTGAGA-3′
(SEQ ID No.
1) and a second primer comprising the sequence of 5′
-TGGTCGCTGATCAGGATGGAA-3′
(SEQ ID No.
2) in said solution;
c denaturing said DNA material to produce two single stranded DNA sequences;
d annealing each of said primer pair to a specific region on each of said single stranded DNA sequences which sequences are complementary to each of said primer sequences;
e extending each of said primer pair on each of said single stranded DNA sequences using each of said single stranded DNA sequences as templates to amplify a specific region of each of said single stranded DNA sequences;
f repeating said steps of denaturing said DNA material to produce two single stranded DNA sequences;
annealing each of said primer pair to a specific region on each of said single stranded DNA sequences which sequences are complementary to each of said primer sequences;
extending each of said primer pair on each of said single stranded DNA sequences using each of said single stranded DNA sequences as templates to amplify a specific region of each of said single stranded DNA sequences; and
g detecting the presence of said specific regions from said DNA material wherein said specific regions have been amplified following said steps of c, d and e and wherein the presence of said specific regions from said DNA material is indicative of male genomic material in said DNA material.
-
Specification