Serum free medium for chondrocyte cells

• US 6,617,159 B1
• Filed: 06/11/2001
• Issued: 09/09/2003
• Est. Priority Date: 11/09/1998
• Status: Expired due to Fees

• Current Assignee: Consorzio Per La Gestione Del Centro Di Biotechnologie Avanzate
• Original Assignee: Consorzio Per La Gestione Del Centro Di Biotechnologie Avanzate, Istituto Nazionale Per La Ricerca Sul Cancro
• Inventors: Cancedda, Ranieri, Dozin, Beatrice
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Ware, Deborah K.
• Application Number:
  US09/831,161
• Time in Patent Office: 820 Days
• Field of Search: 424/93.7, 435/325, 435/389, 435/395, 435/404, 435/407, 435/366
• US Class Current: 435/325
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/36 Lipids
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/125 Stem cell factor [SCF], c-kit ligand [KL]
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

Serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum free medium for growth of chondrocytes includes a serum free composition comprising FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin and dexamethasone. Further the composition comprises EGF, PDGFbb, insulin and albumin. A method for growing chondrocytes in a serum free medium comprising the composition is also provided. Also provided for mesenchymal stem cell growth, is a serum free medium which includes a composition comprising FGF-2, LIF, SCF, pantotenate, biotin and selenium and method, therefore.

Claims

1. A serum-free culture medium for chondrocytes, comprising FGF-2, linoleic acid, ascorbic acid, β

-mercaptoethanol, transferrin and dexamethasone.

2. The serum-free culture medium according to claim 1, further comprising EGF. PDGFbb, insulin and albumin.

3. The serum-free culture medium according to claim 1 or 2, further comprising a minimum essential medium.

4. A method for culturing chondrocytes, which comprises growing cells in a serum-free composition comprising FGF-2, linoleic acid, ascorbic acid, β

-mercaptoethanol, transferrin and dexamethasone.

5. The method according to claim 4, wherein the serum-free composition further comprises EGF, PDGFbb, insulin and albumin.

6. The method according to claim 4 or 5, wherein the serum-free composition further comprises a minimum essential medium.