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Methods for analysis of gene expression

  • US 6,618,679 B2
  • Filed: 01/27/2001
  • Issued: 09/09/2003
  • Est. Priority Date: 01/28/2000
  • Status: Expired due to Term
First Claim
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1. A method for analyzing gene expression comprising:

  • a) obtaining a plurality of target sequences, wherein the plurality of target sequences comprises cDNA;

    b) multiplex amplifying said plurality of target sequences, wherein multiplex amplifying comprises combining the plurality of target sequences, a plurality of target-specific primers, and one or more universal primers, and wherein the universal primer is provided in an excess concentration relative to the target-specific primer, thereby producing a plurality of amplification products;

    c) separating one or more members of the plurality of amplification products;

    d) detecting one or more members of the plurality of amplification products, thereby generating a set of gene expression data;

    e) storing the set of gene expression data in a database; and

    f) performing a comparative analysis on the set of gene expression data, thereby analyzing the gene expression.

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