Electrochemical coagulation assay and device
First Claim
1. A method for detecting a change in the viscosity of a fluid sample, said method comprising:
- (a) introducing a sample into an electrochemical cell comprising a redox couple and oppositely spaced apart working and reference electrodes;
(b) applying an electric potential to said reaction cell to produce a peak in current, followed by a steady state current between said oppositely spaced apart electrodes;
(c) detecting a change from said steady state current; and
(d) relating said change in steady state current to a change in viscosity of said fluid sample.
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Abstract
Methods and devices for electrochemically detecting a change in the viscosity of a fluid are provided. In the subject methods, a fluid sample is introduced into an electrochemical cell having oppositely spaced apart working and reference electrodes. An electric potential is applied to the cell to first achieve a steady state cell current. A decrease in the steady state cell current is then detected and related to a change in viscosity of the sample. In many embodiments, the sample is blood and the change in viscosity is related to the onset of coagulation in the blood sample, and often the PT of the blood sample. Also provided are test strips, kits thereof and meters for use in practicing the subject methods.
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Citations
11 Claims
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1. A method for detecting a change in the viscosity of a fluid sample, said method comprising:
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(a) introducing a sample into an electrochemical cell comprising a redox couple and oppositely spaced apart working and reference electrodes;
(b) applying an electric potential to said reaction cell to produce a peak in current, followed by a steady state current between said oppositely spaced apart electrodes;
(c) detecting a change from said steady state current; and
(d) relating said change in steady state current to a change in viscosity of said fluid sample. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for detecting the onset of coagulation of a blood sample, said method comprising:
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(a) introducing said blood sample into an electrochemical cell comprising;
(i) oppositely spaced apart working and reference electrodes; and
(ii) a reagent mixture comprising a redox couple;
(b) applying an electric potential to said reaction cell to produce a peak in current, followed by a steady state current between said oppositely spaced apart electrodes;
(c) detecting a change from said steady state current; and
(d) relating said change in steady state current to the onset of coagulation in said blood sample. - View Dependent Claims (8, 9, 10, 11)
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Specification