Methods and compositions to assess oxidative brain injury

• US 6,620,800 B1
• Filed: 06/29/1999
• Issued: 09/16/2003
• Est. Priority Date: 06/29/1998
• Status: Expired due to Fees

• Current Assignee: Vanderbilt University
• Original Assignee: Vanderbilt University
• Inventors: Roberts, L. Jackson II
• Primary Examiner(s): Priebe, Scott D.
• Assistant Examiner(s): WOITACH, JOSEPH T
• Application Number:
  US09/342,813
• Time in Patent Office: 1,540 Days
• Field of Search: 435/1, 514/1, 514/125, 514/183, 514/214, 514/762, 514/772.3, 514/116, 514/117, 514/119, 514/215
• US Class Current: 514/115
• CPC Class Codes: G01N 33/88 involving prostaglandins or their receptors

Abstract

A method to assess oxidative stress in vivo includes the steps of measuring an amount of neuroprostanes in a biological sample before the ex vivo development of neuroprostanes in a sample, comparing the measured amount of neuroprostanes with a control and assessing oxidative stress in vivo based on this comparison. There is also provided a marker for oxidated stress by an increase of neuroprostanes in a biological sample compared to a control sample. A diagnostic tool for determining the presence of a neurodegenerative disease provides for determining an increased amount of neuroprostanes in a biological sample compared to that of a control sample.

Claims

1. A marker for oxidative stress comprising isolated and purified neuroprostanes, which increase in a biological sample compared to a control sample during oxidative stress.

2. The marker according to claim 1, wherein said neuroprostanes is F₂-neuroprostane.

3. The marker according to claim 1, wherein said neuroprostanes is E₂-neuroprostane.

4. The marker according to claim 1, wherein said neuroprostanes is D₂-neuroprostane.

5. An isolated and purified neuroprostane formed by a process selected from the group consisting of beta oxidation, omega oxidation double bond reduction, dehydrogenation of side chain hydroxyl groups and reduction of a ring carbonyl to a hydroxyl group.