Flexible assembly for transporting sample fluids into a mass spectrometer

• US 6,621,076 B1
• Filed: 04/30/2002
• Issued: 09/16/2003
• Est. Priority Date: 04/30/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Killeen, Kevin Patrick, van de Goor, Tom A.
• Primary Examiner(s): NGUYEN, KIET TUAN
• Application Number:
  US10/136,825
• Time in Patent Office: 504 Days
• Field of Search: 250/288, 250/281, 250/282, 210/198.2, 210/656, 204/600, 204/601, 204/500, 204/501, 422/69, 422/70
• US Class Current: 250/288
• CPC Class Codes: H01J 49/04 Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

The invention relates to a device for transporting sample fluids into a mass spectrometer. The device comprises a well plate comprising a plurality of wells, a fluid transporting assembly, and a mass spectrometer interface. The fluid-transporting assembly is comprised of a plurality of fluid-transporting conduits, each extending from an inlet port to an outlet port, wherein the assembly exhibits sufficient flexibility to allow movable positioning of the outlet ports with respect to the inlet ports. Each inlet port of the fluid-transporting assembly is positioned in fluid communication with a different well of the well plate to form a plurality of flow paths. Each flow path originates at a well and travels, in succession, through the conduit inlet port, the conduit, the conduit outlet port, and the mass spectrometer interface. Fluids emerging from the mass spectrometer interface may be introduced into a mass spectrometer. The invention also provides a method for transporting sample fluids into a mass spectrometer.

Claims

1. A device for transporting a sample fluid into a mass spectrometer, the device comprising:

a well plate comprising a plurality of wells, wherein each well is defined by an interior surface extending downwardly from an opening at an upper surface of the well plate;

a fluid-transporting assembly comprising a plurality of fluid-transporting conduits, each extending from an inlet port to an outlet port, wherein the assembly exhibits sufficient flexibility to allow movable positioning of the outlet ports with respect to the inlet ports;

a mass spectrometer interface in fluid communication with the outlet ports of the fluid-transporting assembly,

wherein each inlet port of the fluid transporting assembly is positioned in fluid communication with a different well of the well plate, and each well represents the origin of a flow path that travels, in succession, through a well plate opening, an inlet port, a fluid-transporting conduit, an outlet port, and the mass spectrometer interface.

2. The device of claim 1, wherein the well openings are coplanar.

3. The device of claim 1, wherein the well plate comprises at least 96 wells.

4. The device of claim 3, wherein the well plate comprises at least 384 wells.

5. The device of claim 4, wherein the well plate comprises at least 1536 wells.

6. The device of claim 1, wherein the fluid-transporting assembly comprises a substrate and a cover plate arranged in fluid-tight relationship over the substrate surface, wherein the fluid-transporting conduits are each defined by a channel formed in the substrate surface in combination with the cover plate.

7. The device of claim 6, wherein the substrate, the cover plate, or both are comprised of a polymeric material.

8. The device of claim 7, wherein the polymeric material is a biofouling-resistant material.

9. The device of claim 8, wherein the biofouling-resistant material is polyimide.

10. The device of claim 6, wherein the fluid-transporting conduits are parallel to each other.

11. The device of claim 6, wherein the fluid-transporting conduits are substantially identically sized.

12. The device of claim 6, further comprising a plurality of processing chambers, each in fluid communication with a conduit of the fluid-transporting assembly, wherein the processing chambers are downstream from the well plate and upstream from the mass spectrometer interface.

13. The device of claim 12, wherein the processing chambers are located between the substrate and cover plate and communicate the conduits downstream from the inlet ports and upstream from the outlet ports.

14. The device of claim 6, wherein the mass spectrometer interface is formed from a portion of the cover plate.

15. The device of claim 14, wherein the mass spectrometer interface is formed from a portion of the substrate.

16. The device of claim 6, wherein the mass spectrometer interface is formed from a portion of the substrate.

17. The device of claim 1, further comprising a plurality of processing chambers, each in fluid communication with a conduit of the fluid-transporting assembly, wherein the processing chambers are downstream from the well plate and upstream from the mass spectrometer interface.

18. The device of claim 1, wherein the mass spectrometer interface comprises an electrospray nozzle.

19. The device of claim 18, wherein the mass spectrometer interface comprises a plurality of electrospray nozzles.

20. The device of claim 19, wherein the mass spectrometer interface comprises the same number of electrospray nozzles as the number of fluid-transporting conduits of the fluid-transporting assembly.

21. The device of claim 18, further comprising a nebulizing means for nebulizing fluid emerging from the electrospray nozzle.

22. The device of claim 1, wherein the mass spectrometer interface comprises electrically conductive material.

23. The device of claim 22, wherein electrically conductive material is a metallization layer on a surface of the mass spectrometer interface.

24. The device of claim 23, wherein the metallization layer is on an interior surface of the mass spectrometer interface.

25. The device of claim 23, wherein the metallization layer is on an exterior surface of the mass spectrometer interface.

26. The device of claim 22, wherein the electrically conductive material is electrically connected to ground.

27. The device of claim 22, further comprising a potential generation means electrically connected to the conductive material.

28. The device of claim 1, wherein the mass spectrometer interface is attached to the fluid-transporting assembly.

29. The device of claim 1, further including a motive means to transport sample fluid from each well upwardly through the fluid-transporting conduit in fluid communication therewith.

30. The device of claim 29, wherein the motive means comprises a means for applying a voltage differential to induce electrokinetic flow.

31. The device of claim 29, wherein the motive means comprises a means for pressurizing at least one of the wells of the well plate.

32. A mass spectrometric analytical device comprising:

a well plate comprising a plurality of wells, wherein each well is defined by an interior surface extending downwardly from an opening at an upper surface of the well plate;

a fluid-transporting assembly comprising a substrate having a plurality of microchannels formed in a surface thereof, and

a cover plate arranged in fluid-tight relationship over the substrate surface, the cover plate and the microchannels together defining a plurality of fluid-transporting conduits, each extending from an inlet port to an outlet port;

a mass spectrometer interface in fluid communication with the outlet ports of the fluid-transporting assembly, wherein each inlet port of the fluid transporting assembly is positioned in fluid communication with a different well of the well plate, and each well represents the origin of a flow path that travels, in succession, through a well plate opening, an inlet port, a fluid-transporting conduit, an outlet port, and the mass spectrometer interface; and

a mass spectrometer inlet opening in fluid-receiving relationship to the mass spectrometer interface;

wherein the fluid-transporting assembly is arranged such that direction of the flow path from the wells to the fluid-transporting assembly differs from the direction of the flow path from the mass spectrometer interface to the mass spectrometer inlet opening.

33. A method for transporting a sample fluid to a mass spectrometer, comprising:

(a) providing a mass spectrometer interface and a fluid-transporting assembly comprising a plurality of fluid-transporting conduits, each extending from an inlet port to an outlet port and exhibiting sufficient flexibility to allow movable positioning of the outlet port with respect to the inlet port, wherein at least one outlet port is in fluid communication with the mass spectrometer interface;

(b) placing each inlet port of the fluid transporting assembly in fluid communication with a different well of a well plate, wherein the well plate comprises a plurality of wells each containing a sample fluid and further wherein each well is defined by an interior surface extending downwardly from an opening at an upper surface of the well plate;

(c) positioning the mass spectrometer interface so that sample fluid from the well plate is introduced into an inlet port of a mass spectrometer;

(d) applying a motive force to transport sample fluid from a selected well of the well plate through the opening of the selected well, such that the conduit is in fluid communication with the selected well, the mass spectrometer interface, and the inlet port of the mass spectrometer, wherein the direction in which the sample fluid is transported through the opening of the selected well is different from the direction in which the sample fluid is transported through the inlet port of the mass spectrometer.

34. The method of claim 33, wherein step (d) is repeated for a different well of the well plate.

35. The method of claim 33, wherein step (d) is repeated for all wells of the well plate.

36. The method of claim 33, further comprising during step (d), (d′

) processing the sample fluid within the fluid-transport assembly.