Dual function safety and calibration accessory for raman and other spectroscopic sampling

• US 6,621,574 B1
• Filed: 05/25/2000
• Issued: 09/16/2003
• Est. Priority Date: 05/25/2000
• Status: Expired due to Fees

• Current Assignee: Inphotonics, Inc. (EIC Laboratories, Inc.)
• Original Assignee: Inphotonics, Inc. (EIC Laboratories, Inc.)
• Inventors: Forney, Robert W., Kawai, Nancy T.
• Primary Examiner(s): Smith, Zandra V.
• Assistant Examiner(s): STOCK JR, GORDON J
• Application Number:
  US09/578,975
• Time in Patent Office: 1,209 Days
• Field of Search: 356/301, 356/386, 356/243--24, 356/244, 250/252.1, 359/506, 359/894, 73/10.1
• US Class Current: 356/301
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0232 using shutters
• CPC Class Codes: G01J 3/0235 using means for replacing an element by another, for replacing a filter or a grating
• CPC Class Codes: G01J 3/0297 Constructional arrangements for removing other types of optical noise or for performing calibration
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 21/276 with alternation of sample and standard in optical path
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

This invention relates to an accessory for incorporation into Raman and other spectroscopic instruments for combining shuttering and spectroscopic calibration functions. The accessory is comprised of an assembly with at least two positions that may be inserted into the light path, one position allowing light to be directed to and from the sample, and another position containing a reference standard that blocks the beam and provides a shuttering function. When the light strikes the reference standard, a reference spectrum is produced that may be used to calibrate the spectrograph. The device is especially useful in combination with compact sampling accessories for Raman spectroscopy.

Claims

1. An assembly for obtaining calibrated Raman spectra over optical fibers and for ensuring eye safety said assembly comprising:

a) a Raman sampling probe at the terminus of optical fibers for conducting monochromatic light to a sample and/or for returning light scattered off the sample to a spectrograph,

said monochromatic light exiting the distal end of the probe, illuminating the sample, and the light scattered from the sample also collected at the distal end of the probe in a backscattering geometry, and

b) a moveable aperture in the optical path between the distal end of the sampling probe and the sample with at least two mechanically interchangeable positions;

a first position containing an opening or transmissive optical element permitting the monochromatic light to illuminate the sample and backscattered light to be collected by the probe and returned to the spectrograph to produce a sample spectrum, and

a second position blocking light to the sample and to the eye and further placing in the optical path an optical standard that delivers to the probe a standard spectrum which is returned to the spectrograph for instrument calibration.

2. The assembly of claim 1 wherein the optical standard is a luminescent standard that when illuminated with the monochromatic light produces a luminescence spectrum with known spectral distribution of intensity versus wavelength.

3. The assembly of claim 2 wherein the luminescent standard is green bottle glass.

4. The assembly of claim 1 wherein the optical standard is a Raman scattering material that when illuminated with the light source produces a Raman spectrum with known spectral distribution of Raman peak wavelengths.

5. The assembly of claim 4 in which the Raman scattering material standard is Teflon.

6. The assembly of claim 1 wherein the optical standard is a combination of a standard luminescent material and a standard Raman scattering material.

7. The assembly of claim 1 wherein the optical standard is a light absorbing material with negligible optical scattering or emission.

8. The assembly of claim 1 wherein the optical standard is an emissive light source with a known spectral distribution.

9. The assembly of claim 1 wherein the moveable aperture is a wheel with rotational to positions for introducing transmissive elements or standards in the light path.

10. The assembly of claim 1, wherein the moveable aperture is a sliding bar to position said aperture or standards in the optical path.

11. The assembly of claim 1, further incorporating:

an adaptor tube with an insert at one end for an optical extension tube for addressing the sample and another insert at the other end for accepting the distal end of the Raman probe, and in the adaptor tube body separating the two inserts a slot incorporating a slide assembly with at least two positions;

the slide assembly in a first position having on its surface the optical calibration standard;

the slide assembly in a second position having the opening or transmissive optical element.

12. The assembly of claim 11, in which the slot extends completely through the adaptor tube body in order to incorporate a slide assembly with multiple positions for a multiplicity of standards and transmissive optical elements.