System and method for microwave cell lysing of small samples
First Claim
1. A method for microwave assisted cell lysis, said method comprising the steps of:
- providing a sample, said sample including at least a plurality of cells, the volume of said sample comprising up to about 1 mL;
providing microwave radiation comprising at least one of a predetermined frequency, a predetermined wavelength and a predetermined intensity;
placing said sample a wave-guide cavity, said wave-guide cavity having an input port and an output port the dimensions of said wave-guide cavity suitably adapted for effective transmission of microwave radiation in the range of about 18 to about 26 GHz, and applying to said input of said wave-guide cavity said microwave radiation at an input power level of said predetermined frequency for a predetermined period of time, said predetermined frequency in the range of about 18 to about 26 GHz, said microwave radiation exiting said output port at an output power level, said predetermined period of time being sufficient for lysis of at least a portion of said cells.
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Abstract
Efficient cell lysis in small samples, i.e., samples less than one milliliter, is achieved by exposing the sample to microwave radiation in the frequency range of 18 to 26 GHz. The sample containing cells is supported in a wave-guide cavity, and a microwave source provides microwave radiation to the input port of the wave-guide cavity. A computer controls the frequency and source power level of the microwave radiation produced by the microwave source. The computer also monitors the input power level of the microwave radiation at the input port by means of an input power measuring instrument, the output power level at the output port by means of an output power measuring instrument, and the temperature of the sample by means of a thermocouple. In this way, the computer can control the operating parameters to achieve efficient cell lysis.
33 Citations
9 Claims
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1. A method for microwave assisted cell lysis, said method comprising the steps of:
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providing a sample, said sample including at least a plurality of cells, the volume of said sample comprising up to about 1 mL;
providing microwave radiation comprising at least one of a predetermined frequency, a predetermined wavelength and a predetermined intensity;
placing said sample a wave-guide cavity, said wave-guide cavity having an input port and an output port the dimensions of said wave-guide cavity suitably adapted for effective transmission of microwave radiation in the range of about 18 to about 26 GHz, and applying to said input of said wave-guide cavity said microwave radiation at an input power level of said predetermined frequency for a predetermined period of time, said predetermined frequency in the range of about 18 to about 26 GHz, said microwave radiation exiting said output port at an output power level, said predetermined period of time being sufficient for lysis of at least a portion of said cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
determining the intrinsic power loss of said cavity; and
determining the absorptance of said sample.
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8. The method of claim 1, further comprising the steps of:
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measuring said input power level, said output power level and the temperature of said sample; and
adjusting at least one of said input power level and said predetermined frequency as a function of at least one of said input power level, said output power level and the temperature of said sample.
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9. The method of claim 8, wherein said step of adjusting is performed substantially automatically by a processor.
Specification