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Rapid read-out biological indicator

  • US 6,623,955 B2
  • Filed: 05/18/1995
  • Issued: 09/23/2003
  • Est. Priority Date: 11/29/1988
  • Status: Expired due to Term
First Claim
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1. A method of detecting the presence of viable microorganisms after a sterilization cycle comprising the steps of:

  • (a) exposing a source of active enzyme to a sterilization cycle;

    (b) contacting the active enzyme after the sterilization cycle with an aqueous buffer and a substrate which is specific for and reacts with the active enzyme at about 20-70°

    C. to form an enzyme modified product;

    (c) adding a color developer which is specific for and reacts with the enzyme modified product to generate a color in the presence of viable microorganisms at about 20-70°

    C.;

    (d) determining the existence of said color by visual means in about ten minutes to about 60 minutes; and

    (e) correlating the existence of said color with the presence of viable microorganisms.

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