Polarized light scattering spectroscopy of tissue

• US 6,624,890 B2
• Filed: 05/24/2002
• Issued: 09/23/2003
• Est. Priority Date: 01/25/1999
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Feld, Michael S., Dasari, Ramachandra R., Itzkan, Irving, Gurjar, Rajan, Perelman, Lev, Backman, Vadim
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US10/155,194
• Publication Number:
  US 20020171831A1
• Time in Patent Office: 487 Days
• Field of Search: 356/364, 356/365, 356/369, 600/160
• US Class Current: 356/369
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence

Abstract

The present invention relates to the use of polarized light to measure properties of tissue. More particularly, polarized light can be used to detect dysplasia in tissue as the polarization of back-scattered light from such tissues is preserved while the contribution of diffusely scattered light from underlying tissues can be removed. A fiber optic system for delivery and collection of light can be used to measure tissues within the human body.

Claims

1. A fiber optic probe for measuring a surface layer of tissue comprising:

a fiber optic cable optically coupled to a light source, the fiber optic cable delivering light having a plurality of wavelengths from the light source onto the tissue of a patient;

a polarizer optically coupled to the fiber optic cable to polarize incident light directed through the cable onto the tissue;

an analyzer to separate polarization components of light returning from the tissue through the fiber optic cable, the returning light having a component that is periodic as a function of wavelength; and

a detector system that is optically coupled to the analyzer such that the detector system detects the separated polarization components of light including the periodic component, the detector system generating spectral data to determine a size of a structure in the tissue.

2. The probe of claim 1, wherein the polarizer is positioned at a distal end of the fiber optic cable.

3. The probe of claim 1, wherein the analyzer is positioned at a distal end of the fiber optic cable.

4. The probe of claim 1, further comprising an endoscope having a channel through which the probe is inserted.

5. The probe of claim 1, wherein the analyzer comprises a polarizing beam splitter.

6. The probe of claim 1, further comprising a processor that determines a size of tissue cells within the tissue.

7. The probe of claim 1, wherein the detector system generates a second spectrum with detected light, the second spectrum including wavelengths in the range of 300 nm to 1200 nm.

8. The probe of claim 1, wherein the source comprises a broadband light source and a filter wheel.

9. The probe of claim 1, further comprising a spectrometer optically coupled to the fiber optic probe.

10. A fiber optic light delivery and collection system for measuring a surface layer of tissue comprising:

a fiber optic probe housing having a proximal end and a distal end;

at least one central delivery fiber disposed in the fiber optic probe housing, the central delivery fiber coupled to a source through a fiber optic cable, the source providing, a plurality of wavelengths, and a polarizer coupled to the central delivery fiber to polarize incident light directed through the fiber optic cable onto the tissue;

at least one off-center collection fiber disposed in the fiber optic probe housing to collect light returning from the tissue having at least two different polarization components, one component that is periodic as a function of wavelength;

an analyzer coupled to the at least one off-center collection fiber to separate the polarization components of light returning from the tissue; and

a detector system that is optically coupled to the analyzer such that the detector system detects the separated polarization components of light including the periodic component, the detector system generating a spectrum of light having the polarization of the incident light to determine a size of a structure in the tissue.

11. The fiber optic light delivery and collection system of claim 10, wherein the polarizer is positioned at a distal end of the fiber optic cable.

12. The fiber optic light delivery and collection system of claim 10, wherein the polarizer comprises birefringent crystalline material.

13. The fiber optic light delivery and collection system of claim 10, wherein the analyzer is positioned at a distal end of the fiber optic cable.

14. The fiber optic light delivery and collection system of claim 10, further comprising an endoscope having a channel through which the at least one central delivery fiber and at least one off-center collection fiber is inserted.

15. The fiber optic light delivery and collection system of claim 10, wherein the analyzer comprises a polarizing beam splitter.

16. The fiber optic light delivery and collection system of claim 10, further comprising the detector system determining a size of tissue cells within the tissue.

17. The fiber optic light delivery and collection system of claim 10, wherein the detector system generating a second spectrum with detected light, the second spectrum including wavelengths in the range of 300 nm to 1200 nm.

18. The fiber optic light delivery and collection system of claim 10, wherein the source comprises a broadband light source and a filter wheel.

19. The fiber optic light delivery and collection system of claim 10, further comprising a spectrometer optically coupled to the analyzer.