Multiple channel pipetting device

• US 6,627,160 B2
• Filed: 03/20/2001
• Issued: 09/30/2003
• Est. Priority Date: 03/20/2000
• Status: Expired due to Term

• Current Assignee: Brand Gmbh + Co Kg
• Original Assignee: Brand Gmbh + Co Kg
• Inventors: Wanner, Jurgen
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R.
• Application Number:
  US09/811,820
• Publication Number:
  US 20010043885A1
• Time in Patent Office: 924 Days
• Field of Search: 422/100, 738/633.2, 738/64, 738/640.1, 738/641.1, 738/641.6, 738/641.3, 738/641.4, 738/641.7, 738/632.5
• US Class Current: 422/501
• CPC Class Codes: B01L 3/0231 having several coaxial pistons
• CPC Class Codes: G01N 35/1065 Multiple transfer devices

Abstract

A multiple channel pipetting device and pipette shaft, in which each pipette shaft has a circumferential seal receptacle inside, at its upper end in which the circumferential plunger seal is positioned and in which the circumferential plunger seal, at least in the case of a pulled back pipette plunger, definitely remains in the seal receptacle when the pipetted shaft is removed from the shaft carrier.

Claims

1. Multiple channel pipetting device, comprising:

a housing with a transversely arranged shaft carrier and a moveable plunger carrier arranged transversely above the shaft carrier;

a number of plunger receptacles in the shaft carrier and a corresponding number of pipette plungers positioned on the plunger carrier and inserted in the plunger receptacles;

a number of shaft receptacles corresponding to the number of plunger receptacles, said shaft receptacles being inserted in the shaft carrier on an opposite side from the plunger carrier and extending from the plunger receptacles; and

a number of pipette shafts corresponding to the number of shaft receptacles and removably inserted in the shaft receptacles;

wherein each pipette shaft has a cylinder adapted for the pipette plunger and a tip holder on a lower end with an ejection channel arranged therein;

wherein an outer side of an upper end of each cylinder is adapted for attachment in a respective shaft receptacle;

wherein a pipette tip is received on each tip holder; and

wherein each pipette plunger has a circumferential plunger seal which is positioned in a respective circumferential seal receptacle of an inner upper end of each pipette shaft; and

wherein the circumferential plunger seal is mounted so as to remain in the seal receptacle, at least in the case of a pulled back pipette plunger, when the pipette shaft is removed from the shaft carrier.

2. Pipetting device according to claim 1, further comprising an affixing element for affixing the plunger seal in the seal receptacle.

3. Pipetting device according to claim 2, said affixing element is one of a spring ring and a screw-on ring attached to the pipette shaft.

4. Pipetting device according to claim 1, further comprising a stripping ring axially movably arranged in each plunger receptacle in the shaft carrier on a transition to the shaft receptacle, said stripping ring being biased downward in a direction of the shaft receptacle by a spring element;

and wherein the stripping ring affixes the plunger seal in the seal receptacle when the pipette shaft is mounted in the shaft receptacle.

5. Pipetting device according to claim 4, wherein an abutment for the stripping ring is arranged in a lower part of each plunger receptacle in the shaft carrier, toward which the stripping ring is biased by means of the spring element.

6. Pipetting device according to claim 4, wherein the plunger seal is held in the seal receptacle in the pipette shaft by friction coupling.

7. Pipetting device according to claim 1, wherein the plunger seal is a sealing jacket of a low friction material.

8. Pipetting device according to claim 7, wherein the sealing jacket is radially biased by means of an elastomer seal.

9. Pipetting device according to claim 8, wherein the elastomer seal is an O-ring.

10. Pipetting device according to claim 1, wherein each pipette shaft is screwed into the respective shaft receptacle.

11. Pipetting device according to claim 1, wherein each pipette shaft is held in the respective shaft receptacle by a bayonet lock.

12. Pipetting device according to claim 1, wherein each pipette shaft has a circular receiver on the tip holder in which a tip seal for the pipette tip is received.

13. Pipetting device according to claim 12, wherein the tip seal is an elastomer seal.

14. Pipetting device according to claim 13, wherein the tip seal is a V-shaped lip seal of elastomer material.

15. Pipetting device according to claim 1, wherein the pipette shafts are made of a plastic material.

16. Pipette shaft unit for a pipetting device, comprising,

a pipette shaft;

a cylinder adapted to receive a pipette plunger; and

a tip holder on a lower end of the cylinder with an ejection channel arranged therein;

wherein an outer side of an upper end of the cylinder is adapted for attachment in a shaft receptacle;

wherein the tip holder is adapted to receive a pipette tip thereon by pushing;

wherein a circumferential seal receptacle is arranged at an inner side of the upper end of the cylinder; and

wherein a circumferential plunger seal for the pipette plunger is positioned in said seal receptacle.

17. Pipetting shaft according to claim 16, further comprising an affixing element for affixing the plunger seal in the seal receptacle.

18. Pipetting shaft according to claim 17, wherein said affixing element is one of a spring ring and a screw-on ring attached to the pipette shaft.

19. Pipetting shaft according to claim 16, wherein the plunger seal is held in the seal receptacle in the pipette shaft by friction coupling.

20. Pipetting shaft according to claim 16, wherein the plunger seal is a sealing jacket of a low friction material.

21. Pipetting shaft according to claim 20, wherein the sealing jacket is radially biased by means of an elastomer seal.

22. Pipetting shaft according to claim 21, wherein the elastomer seal is an O-ring.

23. Pipetting shaft according to claim 16, wherein each pipette shaft is screwed into the respective shaft receptacle.

24. Pipetting shaft according to claim 16, wherein each pipette shaft is held in the respective shaft receptacle by a bayonet lock.

25. Pipetting shaft according to claim 16, wherein each pipette shaft has a circular receiver on the tip holder in which a tip seal for the pipette tip is received.

26. Pipetting shaft according to claim 25, wherein the tip seal is an elastomer seal.

27. Pipetting shaft according to claim 25, wherein the tip seal is a V-shaped lip seal of elastomer material.

28. Pipetting shaft according to claim 16, wherein the pipette shafts are made of a plastic material.