Vector for gene expression in prokaryotic and eukaryotic systems

• US 6,627,436 B2
• Filed: 10/31/1997
• Issued: 09/30/2003
• Est. Priority Date: 10/31/1997
• Status: Expired due to Fees

• Current Assignee: Stratagene California (Agilent Technologies, Inc.)
• Original Assignee: Stratagene California (Agilent Technologies, Inc.)
• Inventors: Padgett, Kerstien A., Sorge, Joseph A.
• Primary Examiner(s): Brusca, John S.
• Application Number:
  US08/961,888
• Publication Number:
  US 20010016351A1
• Time in Patent Office: 2,160 Days
• Field of Search: 435/69.1, 435/69.7, 435/9.41, 435/320.1, 536/23.4, 536/23.5, 536/24.1
• US Class Current: 435/320.1
• CPC Class Codes: C12N 15/72 Expression systems using regulatory sequences derived from the lac-operon
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2800/108 episomal vectors
• CPC Class Codes: C12N 2830/002 inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
• CPC Class Codes: C12N 2830/15 chimeric enhancer/promoter combination
• CPC Class Codes: C12N 2830/55 from bacteria
• CPC Class Codes: C12N 2840/20 translation of more than one cistron
• CPC Class Codes: C12N 2840/44 being a specific part of the splice mechanism, e.g. donor, acceptor

Abstract

The invention concerns an expression vector that permits expression of genes and fragments thereof in both prokaryotic and mammalian systems. The invention also pertains to derivatives of such vector that contain one or more prokaryotic or eukaryotic (especially mammalian) genes or gene fragments. The invention further pertains to prokaryotic or mammalian cells containing such an expression vector or derivative.

Claims

1. A dual expression vector, said vector comprising:

(a) a cloning site;

(b) a first transcription element comprising a prokaryotic promoter and a second transcription element comprising a eukaryotic promoter, sufficient to permit transcription of a polynucleotide inserted into said cloning site in both a prokaryotic and a eukaryotic cell,

(c) translation elements comprising a Kozak consensus sequence, sufficient to permit translation of an RNA transcript of said polynucleotide in both a prokaryotic and a eukaryotic cell; and

(d) elements sufficient to permit the replication of said vector in prokaryotic cells, wherein said vector mediates the expression of said inserted polynucleotide in both a prokaryotic and a eukaryotic cell, wherein said vector is pdual selected from the group consisting of Clone #2800-9.12, Clone #2995-57.2 and Clone #2995-71AgM-3.