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Methods and compositions for in vivo gene therapy

  • US 6,627,615 B1
  • Filed: 08/11/1998
  • Issued: 09/30/2003
  • Est. Priority Date: 12/17/1991
  • Status: Expired due to Fees
First Claim
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1. A method of introducing a nucleic acid into cells of a mammal, said method comprising introducing the nucleic acid into the mammal systemically, wherein:

  • the nucleic acid is complexed to a lipid carrier comprising cationic lipids and a steroid, the carrier having a mean diameter of less than about 10 microns, resulting in a nucleic acid-lipid carrier complex;

    the nucleic acid-lipid carrier complexes are substantially free of macroaggregates prior to said systemic introduction;

    the nucleic acid to lipid carrier ratio is less than 6;

    1 micrograms nucleic acid to nanomoles cationic lipid; and

    the nucleic acid comprises a DNA expression cassette comprising a promoter and a DNA subsequence encoding a protein.

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