Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses

• US 6,627,748 B1
• Filed: 09/11/2000
• Issued: 09/30/2003
• Est. Priority Date: 09/11/2000
• Status: Active Grant

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Ju, Jingyue, Li, Zengmin, Tong, Anthony, Russo, James J.
• Primary Examiner(s): Baker, Maurie Garcia
• Application Number:
  US09/658,077
• Time in Patent Office: 1,114 Days
• Field of Search: 435/4, 435/6, 435/7.1, 435/DIG.41, 435/91.1, 436/501, 436/518, 536/23.1, 536/24.3, 536/25.32, 530/323, 530/345
• US Class Current: 506/15
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 2533/107 Probe or oligonucleotide ligation
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels
• CPC Class Codes: C40B 40/00 Libraries per se, e.g. arrays, mixtures

Abstract

This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescence emission signature. The invention further provides for the use of said tags in multi-component analyses, including multiplex genetic analyses.

Claims

1. A combinatorial fluorescence energy transfer tag which comprises the structure:

wherein A, B, and C represent different fluorescent molecules which comprise one or more energy transfer donor and one or more energy transfer acceptor,

wherein m and n represent any integer greater than or equal to one, and

wherein fluorescent molecules A, B, and C are separated from each other to produce a unique fluorescence emission signature.

2. The combinatorial fluorescence energy transfer tag of claim 1, wherein the fluorescent molecules are selected from the group consisting of 6-carboxyfluorescein, N,N,N′

,N′

-tetramethyl-6-carboxyrhodamine, and cyanine.

3. An oligonucleotide primer labeled using the combinatorial fluorescence energy transfer tag of claim 1.