Method for screening microcrystallizations for crystal formation
First Claim
1. A method for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising:
- forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and
detecting molecule crystal formation in the drops by taking images of the drops.
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Abstract
A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
122 Citations
84 Claims
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1. A method for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising:
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forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and
detecting molecule crystal formation in the drops by taking images of the drops. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A method for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising:
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forming an array of microcrystallizations, each microcrystallization comprising a hanging drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and
detecting molecule crystal formation in the drops by taking images of the drops. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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44. A method for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising:
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forming an array of microcrystallizations, each microcrystallization comprising a sitting drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and
detecting molecule crystal formation in the drops by taking images of the drops. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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55. A method for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising:
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forming an array of microcrystallizations, each microcrystallization comprising a microcrystallization volume comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the microcrystallization volume having a volume of less than 1 microliter;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the microcrystallization volumes; and
detecting molecule crystal formation in the microcrystallization volumes by taking images of the microcrystallization volumes. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84)
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Specification