Optical measuring head, in particular for automatic chemical or biological reaction analyzer

• US 6,630,108 B1
• Filed: 04/05/2001
• Issued: 10/07/2003
• Est. Priority Date: 10/08/1998
• Status: Expired due to Fees

• Current Assignee: Maxma, Merck Sharp & Dohme Limited (Merck & Co., Inc.)
• Original Assignee: Maxma
• Inventors: Chojnacki, Andre, Vermeulen, Christiaan, Randrianarivo, Jeanet
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/806,869
• Time in Patent Office: 915 Days
• Field of Search: 422/65, 422/82.08, 422/82.09, 356/73, 356/432, 356/440, 356/434
• US Class Current: 422/65
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 2201/0618 Halogene sources
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

An optical measuring head 10 of an automatic chemical or biochemical reaction analyzer comprises a first calorimetric analysis detector 12 controlled by a light source 20 associated to an optic fibre 28 for transmission of the light beam to one of the sides of a reaction analysis plate 18. A photodetector device 37 is arranged on the opposite side to collect the light beam emerging from the cup 16. The light source 20 and the reaction analysis plate 18 are stationary whereas the measuring head 10 is mounted on a U-shaped mobile support 19 framing the parallel opposite faces of the analysis plate 18 and able to be moved by a drive mechanism 48 facing a predetermined cup 16. The optic fibre 28 has one end secured to the fixed light source 20 and an opposite end arranged in a housing 30 of the mobile support 19.

Claims

1. An optical measuring head for an automatic laboratory chemical or biochemical reaction analyser designed to determine the dosing of components contained in samples after mixing with reagents in cups (16, 16a) of a reaction analysis plate (18), said measuring head comprising:

a first colorimetric analysis detector (12, 122) controlled by a light source (20) associated to an optic fibre (28) for transmission of the light beam to one of the sides of the reaction analysis plate (18), coinciding with the optic axis of a predetermined cup, which cup is made of transparent material,

means for focussing the light beam when the latter passes through the reaction mixture (34) in said cup (16),

a photodetector device (37) arranged on the opposite side of the analysis plate (18) to collect the light beam emerging from the cup (16) after this beam has been subjected to an attenuation,

and an electronic processing circuit (46) for processing the signal delivered by the photodetector device (37) to determine the light spectrum or the optical density of the reaction mixture (34),

characterized in that;

the light source (20) and the reaction analysis plate (18) are fixed,

the measuring head (10, 100) comprises a U-shaped mobile support (19) framing the parallel opposite sides of the reaction analysis plate (18), and a drive mechanism (48) of said mobile support to bring the first calorimetric analysis detector (12, 112) to face a predetermined cup,

the optic fibre (28) has one end secured to the fixed light source (20) and an opposite end arranged in a first housing (30) of the mobile support (19).

2. An analyser comprising an optical measuring head according to claim 1, a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

3. The optical measuring head according to claim 1, characterized in that the first housing (30) of the mobile support contains an optical collimator (32) to deliver a parallel light beam and a reference photoelectric detector (36) connected to the electronic circuit (46) to compensate the light flux variations when deformation of the optic fibre occurs (28).

4. The optical measuring head according to claim 3, characterized in that the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.

5. An analyser comprising an optical measuring head according to claim 3, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

6. The optical measuring head according to claim 3, characterized in that the mobile support (19) comprises a second housing (39) located opposite the first housing (30) and containing an optical focussing system (38) optically linked with the photodetector device (37).

7. The optical measuring head according to claim 6, characterized in that the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.

8. An analyser comprising an optical measuring head according to claim 6, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

9. The optical measuring head according to claim 1, characterized in that the optical focussing system (38) is connected to a diffraction network (42) for static wavelength selection.

10. The optical measuring head according to claim 9, characterized in that the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.

11. An analyser comprising an optical measuring head according to claim 9, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

12. The optical measuring head according to claim 9, characterized in that the optical focussing system (38) is connected to the diffraction network (42) by means of a second optic fibre (40).

13. The optical measuring head according to claim 12, characterized in that the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.

14. An analyser comprising an optical measuring head according to claim 12, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

15. An optical measuring head, in particular for an automatic laboratory chemical or biochemical reaction analyser designed to determine the dosing of components contained in samples after mixing with reagents in cups (16, 16a) of a reaction analysis plate (18), said measuring head comprising:

a first colorimetric analysis detector (12, 122) controlled by a light source (20) associated to an optic fibre (28) for transmission of the light beam to one of the sides of the reaction analysis plate (18), coinciding with the optic axis of a predetermined cup, which cup is made of transparent material;

means for focussing the light beam when the latter passes through the reaction mixture (34) in said cup (16);

a photodetector device (37) arranged on the opposite side of the analysis plate (18) to collect the light beam emerging from the cup (16) after this beam has been subjected to an attenuation; and

an electronic processing circuit (46) for processing the signal delivered by the photodetector device (37) to determine the light spectrum or the optical density of the reaction mixture (34),

wherein the light source (20) and the reaction analysis plate (18) are fixed, the measuring head (10, 100) is mounted on a U-shaped mobile support (19) framing the parallel opposite sides of the reaction analysis plate (18) and able to be moved by a drive mechanism (48) to bring the first colorimetric analysis detector (12, 112) to face a predetermined cup, the optic fibre (28) has one end secured to the fixed light source (20) and an opposite end arranged in a first housing (30) of the mobile support (19), and the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.

16. An analyser comprising an optical measuring head according to claim 15, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

17. The optical measuring head according to claim 15, characterized in that the second photometric analysis detector (14) comprises at least one light-emitting diode (52) for emission of a monochromatic light, operating in conjunction with a receiver photodiode (54) on the opposite side of the mobile support (19).

18. An analyser comprising an optical measuring head according to claim 17, and a thermal enclosure designed to receive said analysis plate, characterized in that the bottom face of the analysis plate (18) is kept at a preset temperature by a thermostatic control.

19. An optical measuring head, in particular for an automatic laboratory chemical or biochemical reaction analyser designed to determine the dosing of components contained in samples after mixing with reagents in cups (16, 16a) of a reaction analysis plate (18), said measuring head comprising:

a first colorimetric analysis detector (12, 122) controlled by a light source (20) associated to an optic fibre (28) for transmission of the light beam to one of the sides of the reaction analysis plate (18), coinciding with the optic axis of a predetermined cup, which cup is made of transparent material;

means for focussing the light beam when the latter passes through the reaction mixture (34) in said cup (16);

a photodetector device (37) arranged on the opposite side of the analysis plate (18) to collect the light beam emerging from the cup (16) after this beam has been subjected to an attenuation; and

an electronic processing circuit (46) for processing the signal delivered by the photodetector device (37) to determine the light spectrum or the optical density of the reaction mixture (34),

wherein the light source (20) and the reaction analysis plate (18) are fixed, the measuring head (10, 100) is mounted on a U-shaped mobile support (19) framing the parallel opposite sides of the reaction analysis plate (18) and able to be moved by a drive mechanism (48) to bring the first calorimetric analysis detector (12, 112) to face a predetermined cup, the optic fibre (28) has one end secured to the fixed light source (20) and an opposite end arranged in a first housing (30) of the mobile support (19), the bottom face of the analysis plate (18) is confined in a closed enclosure (56) kept at a preset temperature by a thermostatic control and the enclosure (56) is provided with a deformable flexible wall (58) allowing the mobile support (19) to move under the analysis plate (18).

20. An optical measuring head, in particular for an automatic laboratory chemical or biochemical reaction analyser designed to determine the dosing of components contained in samples after mixing with reagents in cups (16, 16a) of a reaction analysis plate (18), said measuring head comprising:

a first colorimetric analysis detector (12, 122) controlled by a light source (20) associated to an optic fibre (28) for transmission of the light beam to one of the sides of the reaction analysis plate (18), coinciding with the optic axis of a predetermined cup, which cup is made of transparent material;

means for focussing the light beam when the latter passes through the reaction mixture (34) in said cup (16);

a photodetector device (37) arranged on the opposite side of the analysis plate (18) to collect the light beam emerging from the cup (16) after this beam has been subjected to an attenuation

a second sensor having a row of a plurality of light-emitting diodes (52) and associated with receiver diodes; and

an electronic processing circuit (46) for processing the signal delivered by the photodetector device (37) to determine the light spectrum or the optical density of the reaction mixture (34),

wherein the light source (20) and the reaction analysis plate (18) are fixed, the measuring head (10, 100) is mounted on a U-shaped mobile support (19) framing the parallel opposite sides of the reaction analysis plate (18) and able to be moved by a drive mechanism (48) to bring the first colorimetric analysis detector (12, 112) to face a predetermined cup, the optic fibre (28) has one end secured to the fixed light source (20) and an opposite end arranged in a first housing (30) of the mobile support (19), and the measuring head (10) is equipped with a second photometric analysis detector (14) arranged on the mobile support (19) to perform an opacimetry measurement.