Micro-system with multiple points for chemical or biological analysis

• US 6,630,359 B1
• Filed: 01/31/2001
• Issued: 10/07/2003
• Est. Priority Date: 07/31/1998
• Status: Expired due to Fees

• Current Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Original Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Inventors: Clerc, Jean-Frédéric, Caillat, Patrice
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): Simkovic, Viktor
• Application Number:
  US09/744,252
• Time in Patent Office: 979 Days
• Field of Search: 438/1, 435/6, 422/50, 422/100
• US Class Current: 438/1
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00713 Electrochemical synthesis
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A chemical or biological analysis multi-point micro-system including a structure equipped with micro-wells, each micro-well being intended to receive a reagent coupled with a conductive polymer. Each micro-well includes a reception electrode on which the reagent is fixed by the conductive polymer with which it is coupled. Each micro-well also includes a counter-electrode arranged so as to be able to apply, in a volume of the micro-well, an electric field between its counter-electrode and its reception electrode. The structure further enables the simultaneous connection of all the reception electrodes to a first electric potential and enables the simultaneous connection of all the counter-electrodes to a second electric potential to be able to set up the electric field.

Claims

1. A process to produce a chemical or biological analysis multipoint micro-system, comprising steps of:

providing a plurality of micro-wells each configured to receive a reagent coupled with a conductive copolymer, each of the plurality of micro-wells including a reception electrode on which the reagent is to be fixed by the conductive polymer, and a counter-electrode positioned to apply an electric field between the counter-electrode and reception electrode sufficient to copolymerize and fix said conductive polymer with the reagent on the reception electrode, means for simultaneously connecting all the reception electrodes, and means for simultaneously connecting all the counter-electrodes to a second electric potential to set up said electric field;

coupling the reagent with the conductive polymer monomer;

depositing an electrolytic carrier solution containing a mixture of said reagent coupled with said conductive polymer monomer in at least one micro-well of the plurality of micro-wells;

applying an electric field between the reception electrode and the counter-electrode to copolymerise and fix, in the micro-well where the electrolytic solution has been deposited, said conductive polymer with the reagents on the reception electrode; and

rinsing the micro-wells of the structure to eliminate the remaining carrier solution.

2. A process according to claim 1, wherein the coupling, depositing and applying steps are repeated as many times as required to deposit the different reagents in different micro-wells.

3. A chemical or biological analysis multi-point micro-system comprising:

a plurality of micro-wells each configured to receive a reagent coupled with a conductive copolymer, each of the plurality of micro-wells including a reception electrode on which the reagent is to be fixed by the conductive polymer, and a counter-electrode positioned to apply an electric field between the counter-electrode and reception electrode sufficient to copolymerize and fix said conductive polymer with the reagent on the reception electrode;

means for simultaneously connecting all the reception electrodes; and

means for simultaneously connecting all the counter-electrodes to a second electric potential to set up said electric field.

4. A chemical or biological analysis multi-point micro-system according to claim 3, wherein the structure comprises a passive substrate, one face of which is coated with a first conductive layer itself coated with a first layer of insulating material, the first coat of insulating material comprising said micro-wells revealing the first conductive layer which forms said reception electrodes, the first layer of insulating material supporting a second conductive layer forming a common counter-electrode.

5. A chemical or biological analysis multi-point micro-system according to claim 3, wherein the structure comprises an active substrate, one face of which comprises said reception electrodes and is coated with a first layer of insulating material comprising said micro-wells, the base of which corresponds to the reception electrodes, the first layer of insulating material supporting a conductive layer forming a common counter-electrode, multiplexing means being provided to connect all the reception electrodes simultaneously.

6. A chemical or biological analysis multi-point micro-system according to claims 4, wherein a second layer of insulating material covers the conductive layer forming the counter-electrode to embed it.

7. A chemical or biological analysis multi-point micro-system according to claim 6, wherein the second layer of insulating material supports a conductive layer used as a reference pseudo-electrode.

8. A chemical or biological analysis multi-point micro-system according to claim 5, wherein a second layer of insulating material covers the conductive layer forming the counter-electrode to embed it.

9. A chemical or biological analysis multi-point micro-system according to claim 8, wherein the second layer of insulating material supports a conductive layer used as a reference pseudo-electrode.

10. A chemical or biological analysis multi-point micro-system comprising:

a substrate;

a plurality of micro-wells formed on the substrate and each configured to receive a reagent coupled with a conductive polymer, each of the micro-wells including a reception electrode and a counter-electrode positioned to generate an electric field between the counter-electrode and reception electrode sufficient to copolymerize and fix said conductive polymer with the reagent on the reception electrode;

first connecting means for connecting all of the reception electrodes simultaneously; and

second connecting means for connecting all of the counter-electrodes simultaneously to a second electric potential to set up the electric field.

11. A chemical or biological analysis micro-system according to claim 10, wherein:

the substrate comprises a passive substrate, one face of which is coated with a first conductive layer coated with a first layer of insulating material;

the first layer of insulating material forms said micro-wells revealing the first conductive layer and supports a second conductive layer forming a common counter-electrode; and

the first conductive layer forms said reception electrodes.

12. A chemical or biological analysis micro-system according to claim 10, wherein:

the substrate comprises an active substrate, one face of which comprises said reception electrodes and is coated with a first layer of insulating material;

the first layer of insulating material forms said micro-wells and supports a conductive layer forming a common counter-electrode;

the reception electrodes forms bases of said micro-wells, respectively; and

the first connecting means is multiplexing means provided to connect all of the reception electrodes simultaneously.

13. A chemical or biological analysis micro-system according to claims 11, further comprising a second layer of insulating material covering and embedding the conductive layer forming the counter-electrode.

14. A chemical or biological analysis micro-system according to claim 13, wherein the second layer of insulating material is configured to support a conductive layer for a reference pseudo-electrode.

15. A chemical or biological analysis micro-system according to claim 12, further comprising a second layer of insulating material covering and embedding the conductive layer forming the counter-electrode.

16. A chemical or biological analysis micro-system according to claim 15, wherein the second layer of insulating material is configured to support a conductive layer for a reference pseudo-electrode.

17. A chemical or biological analysis micro-system according to claim 11, wherein the first layer of insulating material comprises a photosensitive polymer film.

18. A chemical or biological analysis micro-system according to claim 12, wherein the first layer of insulating material comprises a photosensitive polymer film.

19. A chemical or biological analysis micro-system according to claim 11, wherein the first and second conductive layers comprise a metal selected from the group consisting of chromium, gold, and platinum.

20. A chemical or biological analysis micro-system according to claim 12, wherein the conductive layer comprises a metal selected from the group consisting of chromium, gold, and platinum.