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Chimeric mice that are produced by microcell mediated chromosome transfer and that retain a human antibody gene

  • US 6,632,976 B1
  • Filed: 03/02/1998
  • Issued: 10/14/2003
  • Est. Priority Date: 08/29/1995
  • Status: Expired due to Term
First Claim
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1. A method for producing a chimeric mouse wherein at least some mouse cells retain a foreign chromosome(s) or a fragment(s) thereof, which method comprises:

  • 1) preparing a microcell retaining a foreign chromosome(s) or a fragments thereof that comprise a human antibody gene;

    2) transferring the foreign chromosome(s) or fragment(s) thereof into a pluripotent mouse cell by fusion with the microcell;

    3) transferring the pluripotent mouse cell into a mouse embryo;

    4) transferring the mouse embryo into a uterus or oviduct of a pseudo-pregnant female mouse; and

    5) allowing said mouse embryo to develop into said chimeric mouse.

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