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Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis

  • US 6,635,427 B2
  • Filed: 08/10/2001
  • Issued: 10/21/2003
  • Est. Priority Date: 08/11/2000
  • Status: Expired due to Term
First Claim
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1. A fluorescence-based probe system for analyzing a target nucleic acid consisting essentially ofa single-labeled polynucleotide comprising a sequence generally complementary to a locus of the nucleic acid and a fluorescent label attached to a terminal nucleotide, wherein the terminal nucleotide is a base analog, whereby upon hybridization of the single-labeled polynucleotide to the locus of the nucleic acid the fluorescent label is positioned near a residue of the target nucleic acid with a resultant increase in fluorescent intensity of the fluorescent label.

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