Heating specimen carriers

• US 6,635,492 B2
• Filed: 03/23/2001
• Issued: 10/21/2003
• Est. Priority Date: 01/25/1996
• Status: Expired due to Term

• Current Assignee: BJS Company Ltd. (BJS Group LLC)
• Original Assignee: BJS Company Ltd. (BJS Group LLC)
• Inventors: Gunter, Ian Alan
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US09/815,696
• Publication Number:
  US 20010019704A1
• Time in Patent Office: 942 Days
• Field of Search: 422/99, 422/102, 435/809, 435/287.2, 435/288.4, 219/435, 219/670, 219/672, 219/674, 366/146, 366/273, 436/174
• US Class Current: 436/174
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A method and apparatus for heating specimens in wells of a metallic specimen carrier. The specimen carrier is heated by applying resistive heating directly to the carrier. An AC source and transformer may be used where the specimen carrier is in series with a single turn secondary winding of the transformer. A magnet may be placed in each well and excited by the heating current to provided a stirring effect.

Claims

1. A method of heating a specimen carrier of the kind comprising a plurality of specimen sites, in which said carrier is in the form of a metallic sheet and the method comprising applying a current to said sheet so as to provide resistance heating of said sheet so as to heat specimens carried by said carrier and in which a thickness of a metallic material of the sheet is thinner than an average thickness at a center of the sheet and thicker than said average thickness at an edge of the sheet in such a way as to promote even temperature distribution during heating.

2. A method according to claim 1 in which the step of applying a current to the sheet comprises the step of applying an alternating current to said sheet to provide said resistance heating, and

wherein a magnet is loosely contained within at least one specimen site and is arranged to be agitated by the alternating current so as to provide a stirring action during the heating.

3. A method according to claim 1 wherein the thickness of the metallic material is thinner than the average thickness towards the center of the sheet and thicker than the average thickness along edges of the sheet running generally parallel to a direction in which current flows during operation.

4. A method according to claim 1 in which the sheet is formed by an electrodeposition process and the differences of thickness are achieved by controlling the electrodeposition process.

5. A method according to claim 1 in which the heating is applied as an alternating current providing resistive heating, and is controlled to provide repeated cycles of heating.

6. A method according to claim 1 in which said metallic sheet is of a metal having high electrical and high thermal conductivity.

7. A method according to claim 6 in which said metallic sheet is of silver.

8. A method according to claim 1 in which said sheet is a metallised plastic tray.

9. A method according to claim 1 in which said sheet is an electro-formed metal tray.

10. A method according to claim 1 in which said metallic sheet includes a plurality of wells to contain a plurality of specimens.

11. Apparatus for carrying out the method of claim 1 comprising a specimen carrier of the kind carrying a plurality of specimen sites, in which said carrier is in the form of a metallic electrically conductive sheet, a power supply, and a transformer having a primary winding connected to said power supply, and a secondary winding including to said conductive sheet and in which a thickness of a metallic material of the sheet is thinner than an average thickness at a center of the sheet and thicker than said average thickness at an edge of the sheet in such a way as to promote even temperature distribution during heating.

12. Apparatus according to claim 11 in which the power supply is arranged to apply an alternating current to said sheet to provide resistance heating, and wherein a magnet is loosely contained within at least one specimen site and is arranged to be agitated by the alternating current so as to provide a stirring action during the heating.

13. Apparatus according to claim 11 in which said secondary winding is a single turn winding.

14. Apparatus according to claims 11 comprising a temperature controller connected to regulate flow of heating current through said secondary winding at a rate which maintains a controlled heating temperature within said specimen carrier.

15. Apparatus according to claim 14 which comprises a fan cooling arrangement arranged to direct cooling air to a rear side of said specimen carrier and operatively connected to said temperature controller.

16. Apparatus according to claim 11 in which said metallic sheet is of a metal having high electrical and high thermal conductivity.

17. Apparatus according to claim 16 in which said metallic sheet is of silver.

18. Apparatus according to claim 11 in which said metallic sheet is a metallised plastic tray.

19. Apparatus according to claim 11 in which said metallic sheet is an electro-formed metal tray.

20. Apparatus according to claim 11 wherein the thickness of the metallic material is thinner than the average thickness towards the center of the sheet and thicker than the average thickness along edges of the sheet running generally parallel to a direction in which current flows during operation.

21. A method of heating a specimen carrier in the form of a metallic sheet and in which a matrix of sample wells is incorporated in the sheet,

which method includes applying an alternating current to said sheet to provide heating of the samples in the wells, and

wherein a magnet is loosely contained within at least one well and is arranged to be agitated by the alternating current so as to provide a stirring action during the heating.

22. A method according to claim 21 in which each well contains a magnet.

23. A method according to claim 21 in which the sheet is of a material of high thermal and high electrical conductivity.

24. A method according to claim 21 in which the sample wells are arranged to incorporate samples directly.

26. A method according to claim 21 in which the sample wells are conical in shape.

27. A method according to claim 26 in which the magnet is a bar magnet and the geometry of the conical section of the sample well constrains the bar to spin about an axis that is not coaxial with, or normal to, the axial dimension of the bar.

28. A method according to claim 21 in which the magnet is coated with a non-reactive material.

25. A method according to 21 in which the sample wells are arranged to carry one of sample pots and test tubes shaped to closely fit within the wells.

29. Apparatus for thermally cycling and stirring samples, the apparatus comprising, a specimen carrier in the form of a metallic sheet, in which sheet a matrix of sample wells is incorporated,

a power supply arrangement for applying an alternating current to said sheet to provide heating of the samples in the wells, and

a magnet loosely contained within at least one well which magnet is arranged to be agitated by the alternating heating current so as to provide a stirring action during heating.

30. Apparatus according to claim 29 in which each well contains a magnet.

31. Apparatus according to claim 29 in which the sheet is of a material of high thermal and high electrical conductivity.

32. Apparatus according to claim 29 in which the sample wells are arranged to incorporate samples directly.

33. Apparatus according to claim 29 in which the sample wells are arranged to carry one of sample pots and test tubes shaped to closely fit within the wells.

34. Apparatus according to claim 29 in which the sample wells are conical in shape.

35. Apparatus according to claim 34 in which the magnet is a bar magnet and the geometry of the conical section of the sample well constrains the bar to spin about an axis that is not coaxial with, or normal to, the axial dimension of the bar.

36. Apparatus according to claim 29 in which the magnet is coated with a non-reactive material.

37. A method of heating a specimen carrier of the kind comprising a plurality of specimen sites, in which said carrier is in the form of a metallic sheet and the method comprising applying a current to said sheet so as to provide resistance heating of said sheet so as to heat specimens carried by said carrier and in which the step of applying a current to the sheet comprises the step of applying an alternating current to said sheet to provide said resistance heating, and wherein a magnet is loosely contained within at least one specimen site and is arranged to be agitated by the alternating current so as to provide a stirring action during the heating.

38. Apparatus for carrying out the method of heating a specimen carrier of the kind comprising a plurality of specimen sites, in which said carrier is in the form of a metallic sheet and the method comprising applying a current to said sheet so as to provide resistance heating of said sheet so as to heat specimens carried by said carrier and further comprising a specimen carrier of the kind carrying a plurality of specimen sites, in which said carrier is in the form of a metallic electrically conductive sheet, a power supply, and a transformer having a primary winding connected to said power supply, and a secondary winding including to said conductive sheet and in which the power supply is arranged to apply an alternating current to said sheet to provide resistance heating, and wherein a magnet is loosely contained within at least one specimen site and is arranged to be agitated by the alternating current so as to provide a stirring action during the heating.