Analysis method and cuvette therefor
First Claim
1. A method for quantitative haemoglobin determination in undiluted whole blood comprising the steps of:
- introducing a sample of undiluted whole blood containing red blood cells having haemoglobin therein by capillary action into a disposable microcuvette having at least one cavity for receiving the sample, the cavity including an essentially non-hygroscopic hemolysing agent in a dry form, the cavity being essentially free of azide and nitrite, whereby the hemolysing agent is dissolved in the whole blood, the agent hemolyses the red blood cells and the haemoglobin contained in the blood cells is released;
performing a first absorption measurement at a wavelength range directly on the hemolysed sample in the cuvette, further conducting a second absorption measurement on the hemolysed sample to compensate for background interference, and analyzing the results of the absorption measurements and determining the concentration of haemoglobin in the whole blood sample.
1 Assignment
0 Petitions
Accused Products
Abstract
A method for hemoglobin determination is described which includes the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.
-
Citations
19 Claims
-
1. A method for quantitative haemoglobin determination in undiluted whole blood comprising the steps of:
-
introducing a sample of undiluted whole blood containing red blood cells having haemoglobin therein by capillary action into a disposable microcuvette having at least one cavity for receiving the sample, the cavity including an essentially non-hygroscopic hemolysing agent in a dry form, the cavity being essentially free of azide and nitrite, whereby the hemolysing agent is dissolved in the whole blood, the agent hemolyses the red blood cells and the haemoglobin contained in the blood cells is released;
performing a first absorption measurement at a wavelength range directly on the hemolysed sample in the cuvette, further conducting a second absorption measurement on the hemolysed sample to compensate for background interference, and analyzing the results of the absorption measurements and determining the concentration of haemoglobin in the whole blood sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
-
- 16. Disposable microcuvette for spectrophotometric determination of haemoglobin in undiluted whole blood, wherein the microcuvette comprises a cavity containing a dried, non-hygroscopic hemolysing agent or a combination of said agents, provided that the cavity is essentially free from azide and nitrite, wherein the hemolysing agent is a salt of deoxycholic acid.
Specification