Stable solid enzyme compositions and methods employing them

• US 6,638,902 B2
• Filed: 02/01/2001
• Issued: 10/28/2003
• Est. Priority Date: 02/01/2001
• Status: Expired due to Term

• Current Assignee: Ecolab USA Incorporated (Ecolab, Inc.)
• Original Assignee: Ecolab, Inc.
• Inventors: Tarara, James J., Bradley, Tareasa Lynn, Swart, Sally K., Chandler, Denise, Rabon, Reid
• Primary Examiner(s): Gupta, Yogendra N.
• Assistant Examiner(s): ELHILO, EISA B
• Application Number:
  US09/773,965
• Publication Number:
  US 20020177541A1
• Time in Patent Office: 999 Days
• Field of Search: 510/161, 510/392, 510/224, 134/1, 134/2, 134/22.14, 134/22.16, 134/22.17, 134/22.19, 134/25.1, 134/25.4, 134/35.42
• US Class Current: 510/392
• CPC Class Codes: C11D 2111/20 Industrial or commercial equipment, e.g. reactors, tubes or engines
• CPC Class Codes: C11D 3/10 Carbonates ; Bicarbonates
• CPC Class Codes: C11D 3/386 Preparations containing enzymes , e.g. protease or amylase
• CPC Class Codes: C11D 3/38627 containing lipase
• CPC Class Codes: C11D 3/38636 containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: C11D 3/38654 containing oxidase or reductase

Abstract

The present invention relates to a solid enzyme cleaning composition in which the enzyme is stable in the presence of mixtures of carbonate and bicarbonate at alkaline pH, and methods employing this composition. The enzyme cleaning composition preferably employs weight ratios of carbonate and bicarbonate to stabilize one or more enzymes in a solid, a concentrate, and/or a use composition, and at temperatures higher than ambient.

Claims

1. A method of cleaning a medical or dental instrument, comprising:

dissolving a solid enzyme cleaning composition in water, the solid enzyme cleaning composition comprising detersive enzyme and a mixture of carbonate and bicarbonate;

the carbonate and bicarbonate being present in a weight ratio of about 0.5;

1 to about 4.75;

1;

contacting the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature wherein said;

the medical or dental instrument comprising forceps, scissor, shear, saw, hemostat, knife, chisel, rongeur, file, nipper, drill, drill bit, rasp, burr, spreader, breaker, clamp, needle holder, carrier, clip, hook, gouge, curette, retractor, straightener, punch, extractor, scoop, keratome, expressor, trocar, dilator, cage, catheter, cannula, plug, stent, arthoscope, or combinations thereof.

2. The method of claim 1, wherein the weight ratio of carbonate to bicarbonate is in the range of about 2:

1 to about 3;

1.

3. The method of claim 2, wherein the composition comprises about 30 to about 50 weight percent carbonate and about 10 to about 30 weight percent bicarbonate.

4. The method of claim 1, wherein the composition further comprises an E-form hydrate.

5. The method of claim 1, wherein the detersive enzyme retains at least about 50% of its initial activity at 120°

F. for at least about 30 minutes after dissolving the composition.

6. The method of claim 1, wherein the detersive enzyme comprises protease, amylase, lipase, cellulase, peroxidase, gluconase, or a combination thereof.

7. The method of claim 1, wherein the composition further comprises nonionic surfactant, builder, and chelating agent.

8. The method of claim 7, wherein the nonionic surfactant comprises nonyl phenol ethoxylate, the builder comprises tripolyphosphate, and the chelating agent comprises amino tri(methylene phosphonic acid) (ATMP).

9. The method of claim 1, wherein the solid enzyme cleaning composition is in the form of pellet, tablet, lozenge, puck, briquette, brick, solid block, or unit dose.

10. The method of claim 1, wherein the solid enzyme cleaning composition comprises plurality of detersive enzymes.

11. The method of claim 1, wherein the solid enzyme cleaning composition comprises metal protecting silicate.

27. The method of claim 1, wherein the saw comprises a bone saw.

28. The method of claim 1, wherein:

contacting the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature comprises;

presoaking the medical or dental instrument in the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature.

29. The method of claim 1, wherein:

contacting the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature comprises;

spraying the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature.

30. The method of claim 1, wherein:

contacting the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature comprises;

machine washing the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature.

31. The method of claim 1, wherein:

contacting the medical or dental instrument with the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature comprises;

ultrasonic treatment of the medical or dental instrument in the dissolved solid enzyme cleaning composition at a temperature at or above ambient temperature.

12. A solid enzyme cleaning composition:

the composition being in the form of pellet, tablet, lozenge, puck, briquette, brick, solid block, or unit dose;

the composition comprising;

detersive enzyme and

a mixture of carbonate and bicarbonate;

the carbonate and bicarbonate being present in a weight ratio of about 3.5;

1 to about 4.75;

1;

wherein the ratio of carbonate to bicarbonate is selected to increase stability of the detersive enzyme in the solid composition at ambient temperature.

13. The composition of claim 12, comprising about 30 to about 50 weight percent carbonate and about 10 to about 30 weight percent bicarbonate.

14. The composition of claim 12, wherein the detersive enzyme retains at least about 50% of its initial activity at 120°

F. for at least about 30 minutes after forming a use composition.

15. The composition of claim 12, further comprising an E-form hydrate.

16. The composition of claim 12, wherein the detersive enzyme comprises protease, amylase, lipase, cellulase, peroxidase, gluconase, or a combination thereof.

17. The composition of claim 16, wherein the detersive enzyme comprises alkaline protease, lipase, amylase, or a combination thereof.

18. The composition of claim 12, further comprising nonionic surfactant, builder, and chelating agent.

19. The composition of claim 18, wherein the nonionic surfactant comprises nonyl phenol ethoxylate, the builder comprises tripolyphosphate, and the chelating agent comprises amino tri(methylene phosphonic acid) (ATMP).

20. The composition of claim 19, comprising about 8 wt-% nonyl phenol ethoxylate, about 18 wt-% tripolyphosphate, about 4 wt-% protease, and about 5 wt-% ATMP.

21. The composition of claim 19, comprising about 8 wt-% nonyl phenol ethoxylate 9.5, about 17 wt-% tripolyphosphate, about 8 wt-% protease, and about 5 wt-% ATMP.

22. The composition of claim 19, comprising about 7.5 wt-% nonyl phenol ethoxylate 9.5, about 20 wt-% tripolyphosphate, about 1 wt-% protease, and about 7 wt-% ATMP.

23. A liquid concentrate composition comprising:

about 0.3 to about 4 wt-% water,

detersive enzyme,

and a mixture of carbonate and bicarbonate;

the carbonate and bicarbonate being present in a weight ratio of about 3.5;

1 to about 4.75;

1.

24. A solid enzyme cleaning composition comprising:

plurality of detersive enzymes, the plurality of detersive enzymes comprising;

protease plus;

amylase, lipase, cellulase, peroxidase, gluconase, or a combination thereof; and

a mixture of carbonate and bicarbonate;

the carbonate and bicarbonate being present in a weight ratio of about 3.5;

1 to about 4.75;

1;

wherein the ratio of carbonate to bicarbonate is selected to increase stability of the plurality of detersive enzymes.

25. The composition of claim 24, wherein the mixture of carbonate and bicarbonate stabilizes the plurality of enzymes in use compositions at use temperatures.

26. The composition of claim 24, wherein the plurality of detersive enzymes comprises:

alkaline protease plus

lipase, amylase, or a combination thereof.