Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation

• US 6,641,708 B1
• Filed: 08/23/1999
• Issued: 11/04/2003
• Est. Priority Date: 01/31/1996
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Yang, Jun, Huang, Ying, Gascoyne, Peter R. C., Becker, Frederick F., Wang, Xiao-Bo
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US09/378,874
• Time in Patent Office: 1,534 Days
• Field of Search: 204/547, 204/643
• US Class Current: 204/547
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: B03C 5/028 using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
• CPC Class Codes: G01N 2030/0035 electrical field
• CPC Class Codes: G01N 2030/0065 Dielectric FFF, i.e. opposing forces dominate hydrodynamic lift forces and steric effects
• CPC Class Codes: G01N 2030/007 programming of driving force carrier programming G01N30/02
• CPC Class Codes: G01N 30/0005 Field flow fractionation

Abstract

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

Claims

1. A method of discriminating matter in a chamber having an inlet port and an outlet port, said chamber defined by a pair of side walls, a top wall and a bottom wall, and an electrode element adapted to said chamber, said chamber having a substantially greater width than thickness, utilizing dielectrophoresis and field flow fractionation, comprising:

introducing a carrier medium including said matter into said inlet port and directing said carrier medium from said inlet port to said outlet port, such that said carrier medium travels through said chamber according to a velocity profile;

applying a programmed voltage signal to said electrode element to create a spatially inhomogeneous electric field which causes a dielectrophoretic force on said matter having components normal to the direction of said carrier medium traveling through said chamber; and

controlling said spatially inhomogeneous electric field to balance said dielectrophoretic force with a gravitational force on said matter to displace said matter to positions within said velocity profile in said carrier medium to discriminate said matter.

2. The method of claim 1, wherein said programmed voltage signal comprises a time dependent amplitude or frequency.

3. The method of claim 1, wherein said programmed voltage signal comprises frequency modulation.

4. The method of claim 1, wherein said programmed voltage signal comprises amplitude modulation.

5. The method of claim 1, wherein said programmed voltage signal comprises a sweeping frequency.

6. The method of claim 1, wherein said programmed voltage signal comprises a series of voltage signals, said voltage signals having different waveforms.

7. The method of claim 6, wherein said different waveforms differ in signal frequency, signal amplitude, frequency modulation, or amplitude modulation.

8. The method of claim 1, wherein said matter travels through said chamber at a velocity proportionate to its displacement within said velocity profile.

9. The method of claim 1, wherein said matter exits from said outlet port at time intervals proportionate to its displacement within said velocity profile.

10. The method of claim 1, wherein said matter exits from said outlet port at positions laterally displaced from said inlet port.

11. The method of claim 1, wherein said method of discriminating matter is in continuous mode.

12. The method of claim 1, wherein said method of discriminating matter is in batch mode.

13. A method of discriminating matter, utilizing dielectrophoresis and field flow fractionation, in a chamber having an inlet port and an outlet port, said chamber defined by a pair of side walls, a top wall and a bottom wall, and an electrode element adapted to said chamber, said chamber having a substantially greater width than thickness, comprising:

introducing a carrier medium from said inlet port into said chamber;

introducing said matter into said inlet port;

introducing a transport fluid into said inlet port and directing said transport fluid from said inlet port to said outlet port, such that said transport fluid travels through said chamber according to a velocity profile;

applying a programmed voltage signal to said electrode element to create a spatially inhomogeneous electric field which causes a dielectrophoretic force on said matter having components normal to the direction of said transport fluid traveling through said chamber; and

controlling said spatially inhomogeneous electric field to balance said dielectrophoretic force with a gravitational force on said matter to displace said matter to positions within said velocity profile in said transport fluid to discriminate said matter.

14. The method of claim 13, wherein said matter travels through said chamber at a velocity proportionate to its displacement within said velocity profile.

15. The method of claim 13, wherein said matter exits from said outlet port at time intervals proportionate to its displacement within said velocity profile.

16. A method of discriminating matter utilizing dielectrophoresis and field flow fractionation in a chamber defined by a pair of side walls, a top wall, and a bottom wall, said method comprising:

introducing a carrier medium including said matter into an inlet port of said chamber and directing said carrier medium from said inlet port toward top and bottom outlet ports coupled to said top and bottom walls, respectively;

applying an electrical signal to an electrode element coupled to said chamber to create a spatially inhomogeneous electric field to generate a dielectrophoretic force on said matter having components normal to the direction of said carrier medium traveling through said chamber;

controlling said spatially inhomogeneous electric field to balance said dielectrophoretic force with a gravitational force on said matter to displace said matter to different heights within said chamber to discriminate said matter;

withdrawing a first portion of said carrier medium from said top outlet port; and

withdrawing a second portion of said carrier medium from said bottom outlet port.

17. The method of claim 16, wherein at least a portion of said matter is withdrawn from said top outlet port.

18. The method of claim 16, wherein at least a portion of said matter is withdrawn from said bottom outlet port.

19. The method of claim 16, wherein said carrier medium is introduced into said inlet port at a first fluid flow rate, and wherein said first portion is withdrawn at a second fluid flow rate, said second fluid flow rate being less than or equal to said first fluid flow rate.

20. The method of claim 16, wherein said carrier medium is introduced into said inlet port at a first fluid flow rate, and wherein said first portion is withdrawn at a second fluid flow rate equal to about one half of said first fluid flow rate.

21. The method of claim 16, wherein said first portion is withdrawn at a first fluid flow rate and wherein said second portion is withdrawn at a second fluid flow rate.

22. The method of claim 21, further comprising varying said first and second fluid flow rates to reduce fluid pressure of at least one of said top or bottom outlet ports.

23. The method of claim 21, further comprising varying said first and second fluid flow rates to further discriminate said matter.

24. The method of claim 16, wherein said first portion or said second portion is withdrawn with a syringe pump.

25. The method of claim 16, wherein said method of discriminating is in continuous mode.

26. The method of claim 16, wherein said method of discriminating is in batch mode.

27. The method of claim 16, wherein said carrier medium travels through said chamber according to a velocity profile such that said carrier medium moves more rapidly at the center of said chamber.

28. The method of claim 27, wherein said velocity profile is parabolic.

29. The method of claim 16, wherein said carrier medium travels through said chamber according to a plug-like profile.

30. The method of claim 16, wherein said electrical signal comprises a programmed voltage signal.

31. The method of claim 16, wherein said chamber has a substantially greater width than thickness.

32. A method of discriminating matter utilizing dielectrophoresis and field flow fractionation in a chamber defined by a pair of side walls, a top wall, and a bottom wall, said method comprising:

introducing a carrier medium including said matter into an inlet port of said chamber and directing said carrier medium from said inlet port toward top and bottom outlet ports coupled to said top and bottom walls, respectively;

applying an electrical signal to an electrode element coupled to said chamber to create a spatially inhomogeneous electric field to generate a dielectrophoretic force on said matter having components normal to the direction of said carrier medium;

controlling said spatially inhomogeneous electric field to balance said dielectrophoretic force with a gravitational force on said matter to displace said matter to different heights within said chamber to discriminate said matter;

withdrawing a first portion of said carrier medium from said top outlet port at a first fluid flow rate; and

withdrawing a second portion of said carrier medium from said bottom outlet port at a second fluid flow rate.

33. The method of claim 32, further comprising controlling said first and second fluid flow rates to further discriminate said matter.

34. The method of claim 32, further comprising detecting discriminated matter with a detector coupled to said chamber.

35. The method of claim 32, wherein said carrier medium is introduced into said inlet port at a third fluid flow rate, and wherein said first fluid flow rate is less than or equal to said third fluid flow rate.

36. The method of claim 32, wherein said carrier medium is introduced into said inlet port at a third fluid flow rate, and wherein said first fluid flow rate is about one half of said third fluid flow rate.

37. The method of claim 32, wherein said method of discriminating is in continuous mode.

38. The method of claim 32, wherein said method of discriminating is in batch mode.

39. The method of claim 32, wherein said electrical signal comprises a programmed voltage signal.

40. An apparatus for discriminating matter, comprising:

a chamber defined by a top and bottom wall;

an outlet port coupled to said chamber;

an inlet port coupled to said chamber and in spaced relation with outlet port;

an electrode element coupled to said chamber and configured to create a spatially inhomogeneous electric field to generate a dielectrophoretic force on said matter; and

two or more sensing electrode elements coupled to said chamber and defining a detector integral with said chamber.