Containment cuvette for PCR and method of use

• US 6,645,758 B1
• Filed: 09/28/1992
• Issued: 11/11/2003
• Est. Priority Date: 02/03/1989
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Inventors: Hinckley, Charles Cullis, Seaberg, Leonard Joseph, Donish, William Harold, Schnipelsky, Paul Nicholas, Wellman, Jeffrey Allen, Findlay, John Bruce
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/951,616
• Time in Patent Office: 4,061 Days
• Field of Search: 422/58, 422/55, 422/61, 422/102, 422/103, 422/81, 422/68.1, 422/100, 435/257, 435/300, 435/301, 435/808, 435/287.2, 435/287.6, 435/288.5, 436/501, 436/165, 436/180, 436/808, 206/219
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A cuvette and a method of use prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

Claims

1. A closed, disposable cuvette for carrying out amplification and detection of nucleic acid material, comprising:

a plurality of compartments defined by walls, including a reaction compartment, said reaction compartment containing nucleic acid material and amplifying reagents, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

at least one detection material being present in at least one of said compartments;

and means for fluidly interconnecting said compartments in prescribed order when pressure is applied to the contents of a compartment;

said compartments all being closed to fluid flow to locations outside of the cuvette;

at least one of said compartments including means at a detection site therein for immobilizing at said site said nucleic acid material for detection after amplification;

whereby detection of amplified nucleic acid material occurs without contamination by the amplified nucleic acid material of other cuvettes for the assay of the same nucleic acid material.

3. A cuvette as defined in claim 1, or 2, wherein said at least one wall comprises transmitting means for rapidly transmitting heat from an external source to the liquid in said reaction compartment, and then back out of said reaction compartment.

4. A cuvette as defined in claim 3, wherein said transmitting means comprises a heat transfer material that forms a wall of said reaction compartment.

5. A cuvette as defined in claim 4, wherein said heat transfer wall has a thermal path length of no more than about 0.3 mm and a thermal resistance of no more than about 5°

C./watt.

6. A cuvette as defined in claim 1, and further including as part of said cuvette, means for pressurizing the contents of at least said reaction compartment to force the contents thereof to transfer out, and a passageway connecting said reaction compartment with said detection site.

7. A cuvette as defined in claim 6, wherein said pressurizing means include a first piston chamber and a first piston in said chamber, fluidly connected to said reaction compartment so that the advance of said piston in said chamber causes pressure to be increased in said reaction compartment.

8. A cuvette as defined in claim 7, and further including a second piston chamber and piston therein, fluidly connected to said detection site so that when said second piston is withdrawn in its chamber, it relieves pressure at said detection site.

9. A cuvette as defined in claim 1 or 2, wherein at least one wall of said compartments is sufficiently flexible as to allow external pressure to compress said compartments to force liquid transfer out of said compartments.

10. A cuvette as defined in claim 9, wherein said compartments and their interconnections are disposed so that their contents can be forced out in a prescribed sequence by applying exterior pressure linearly to said cuvette that advances sequentially along the outside surfaces of the cuvette.

11. A cuvette as defined in claim 1 or 2, wherein said detection site is in said reaction compartment, and said detection material includes a bead comprising magnetizable material.

12. A cuvette as defined in claim 1 or 2, wherein one of said compartments contains water and a passageway extends from said one compartment to all of said other compartments, said one compartment being positioned so as to be the first to be pressurized.

13. A cuvette as defined in claim 1 or 2, wherein said detection site includes a polymer bead attached to a DNA probe.

14. A cuvette as defined in claim 1 or 2, wherein said detection site includes a polymer bead to which is attached avidin.

15. A cuvette as defined in claim 1 or 2, wherein said reaction compartment contains nucleic acid material or amplifying reagents.

26. A cuvette as defined in claim 1, 2, or 16, and further including an extraction compartment positioned fluidly upstream of said reaction compartment, and a filter interposed in the fluid path from said extraction compartment to said reaction compartment, said filter being sized sufficiently to pass DNA therethrough;

whereby DNA extracting agents can be added to the cuvette, along with at least blood cells, and the cellular fragments can be separated from the DNA by said filter so that only the DNA proceeds to said reaction compartment.

2. A closed, disposable cuvette for carrying out amplification and detection of nucleic acid material, comprising:

a plurality of compartments defined by walls, including a reaction compartment, said reaction compartment containing nucleic acid material, polymerase enzyme, primer nucleic acids and nucleotides, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

at least one detection material being present in at least one of said compartments;

and means for fluidly interconnecting said compartments in prescribed order when pressure is applied to at least one of said compartments;

said compartments all being closed to fluid flow to locations outside of the cuvette;

at least one of said compartments including means at a detection site therein for immobilizing said nucleic acid material at said site for detection after amplification;

whereby detection of amplified nucleic acid material occurs without contamination by the amplified nucleic acid material of other cuvettes for the assay of the same nucleic acid material.

16. A closed, disposable cuvette for carrying out amplification and detection of nucleic acid material, comprising:

a plurality of compartments defined by walls, including a reaction compartment, said reaction compartment containing nucleic acid material, TAQ polymerase, primer nucleic acids and nucleotides, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

at least one detection material being present in at least one of said compartments;

and means for fluidly interconnecting said compartments in prescribed order when pressure is applied to at least one of said compartments;

said compartments all being closed to fluid flow to locations outside of the cuvette;

at least one of said compartments including means at a detection site therein for immobilizing at said site said nucleic acid material for detection after amplification;

whereby detection of amplified nucleic acid material occurs without contamination by the amplified nucleic acid material of other cuvettes for the assay of the same nucleic acid material.

27. A cuvette as defined in claim 2 or 16, wherein said detection material includes a probe that contains genetic material that is complementary to a DNA material, and therefore hydridizes with said DNA material when appropriately heated.

28. A cuvette as defined in claim 27, wherein said probe is complementary to the amplified target DNA.

29. A cuvette as defined in claim 27, and further including a positive control probe containing genetic material that is complementary to an amplified DNA that is always present from a blood sample.

30. A cuvette as defined in claim 29, wherein said positive control probe genetic material is complementary to beta-globin.

31. A cuvette as defined in claim 29, wherein said positive control probe is immobilized on a portion of said detection site, in the absence of amplified DNA.

32. A cuvette as defined in claim 27, and further including a negative control probe containing genetic material that is not complementary to any DNA that is expected to be present after amplification of the blood sample.

33. A cuvette as defined in claim 32, wherein said negative control probe is immobilized on a portion of said detection site, in the absence of amplified DNA.

34. A cuvette as defined in claim 27, and further including positive and negative control probes containing, respectively, genetic material complementary to amplified DNA that is always present from the sample, and genetic material that is not complementary to known DNA of the sample.

35. A cuvette as defined in claim 34, wherein said positive control and said negative control probes are immobilized on a portion of said detection site in the absence of any amplified DNA, each of said immobilized site portions being substantially free of the other control probe.

17. Apparatus for amplifying and detecting DNA, comprising

a cuvette containing i) a plurality of compartments defined by walls, and means for interconnecting each of them to at least one other compartment, said compartments including a) at least one reaction compartment for amplifying DNA strands, b) reagents for amplifying and detecting a DNA strand, and c) at least one detection compartment for detecting amplified DNA and including a detection site, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

ii) liquid access means connected only to said at least one reaction compartment for allowing the injection into said reaction compartment of a sample DNA for amplifying;

iii) means for closing off said cuvette against passage of DNA after sample DNA is injected;

and iv) means for moving a detection reagent and a DNA strand into said detection compartment and for immobilizing said DNA strand onto said detection site;

whereby once a DNA sample is injected into the compartments and said access means is closed, the fluid contents of the compartments are contained against contact by the operator and environment during the entire amplification and detection reaction.

18. Apparatus as defined in claim 17, wherein said at least one wall comprises transmitting means for rapidly transmitting heat from an external source to the liquid in said reaction compartment, and then back out of said reaction compartment.

19. Apparatus as defined in claim 18, wherein said transmitting means comprises a heat transfer material that forms a wall of said reaction compartment.

20. Apparatus as defined in claim 19, wherein said heat transfer wall has a thermal path length of no more than about 0.3 mm and a thermal resistance of no more than about 5.0°

C./watt.

21. Apparatus as defined in claim 17, and further including means connected to said reaction compartment for pressurizing the contents of at least said reaction compartment to force the contents thereof to transfer out, and a passageway connecting said reaction compartment with said detection site.

22. Apparatus as defined in claim 21, wherein said pressurizing means include a first piston chamber and a first piston in said chamber, fluidly connected to said reaction compartment so that the advance of said piston in said chamber causes pressure to be increased in said reaction compartment.

23. Apparatus as defined in claim 22, and further including a second piston chamber and piston therein, fluidly connected to said detection site so that when said second piston is withdrawn in its chamber, it relieves pressure at said detection site.

24. Apparatus as defined in claim 17, wherein at least one wall of said compartments is sufficiently flexible as to allow external pressure to compress said compartments to force liquid transfer out of said compartments.

25. Apparatus as defined in claim 24, wherein said compartments and their interconnections are disposed so that their contents can be forced out in a prescribed sequence by applying exterior pressure linearly to said cuvette that advances sequentially along the outside surfaces of the cuvette.

36. Apparatus as defined in claim 17, wherein said detection material includes a probe that contains genetic material that is complementary to an amplified DNA material, and therefore hydridizes specifically with said DNA material when appropriately heated.

37. Apparatus as defined in claim 36, wherein said probe is complementary to the amplified target DNA.

38. Apparatus as defined in claim 36, and further including a positive control probe containing genetic material that is complementary to a DNA that is always present from a blood sample.

39. Apparatus as defined in claim 38, wherein said positive control probe genetic material is complementary to beta-globin.

40. Apparatus as defined in claim 38, wherein said positive control probe immobilized on a portion of said detection site, in the absence of amplified DNA.

43. Apparatus as defined in claim 36, and further including positive and negative control probes containing, respectively, genetic material complementary to a DNA that is always present in the sample, and to genetic material that is not complementary to known DNA of the sample.

44. Apparatus as defined in claim 43, wherein said positive control and said negative control probes are immobilized on a portion of said detection site in the absence of any amplified DNA, each of said immobilized site portions being substantially free of the other control probe.

41. Apparatus as defined in 36, and further including a negative control probe containing genetic material that is not complementary to known DNA of the sample.

42. Apparatus as defined in claim 41, wherein said negative control probe is immobilized on a portion of said detection site, in the absence of amplified DNA.

45. Apparatus for detecting DNA available in a sample in minute quantities, comprising

a cuvette containing i) a plurality of compartments defined by walls, and means for interconnecting each of them to at least one other compartment, said compartments including a) at least one reaction compartment, b) reagents for producing a detectable signal representing a target DNA and including a detection reagent, and c) a detection site for detecting DNA, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

ii) liquid access means connected only to said at least one reaction compartment for allowing the injection into said reaction compartment of a sample DNA;

iii) means for closing off said cuvette against passage of DNA after sample DNA is injected;

and iv) means for moving said detection reagent and a DNA strand to said detection site;

whereby once a DNA sample is injected into the compartments and said access means is closed, the fluid contents of the compartments are contained against contact by the operator and environment during the entire amplification and detection reaction.

46. Apparatus for detecting nucleic acid material available in a sample in minute quantities, comprising

a cuvette containing i) a plurality of compartments defined by walls, and means for interconnecting each of them to at least one other compartment, said compartments including a) at least one reaction compartment, b) reagents for producing a detectable signal representing a target nucleic acid material and including a detection reagent, and c) a detection site for detecting said target material, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment;

ii) liquid access means connected only to said at least one reaction compartment for allowing the injection into said reaction compartment of a sample nucleic acid material;

iii) means for closing off said cuvette against passage of nucleic acid material after sample nucleic acid material is injected;

and iv) means for moving said detection reagent and a nucleic acid material strand to said detection site;

whereby once a nucleic acid material sample is injected into the compartments and said access means is closed, the fluid contents of the compartments are contained against contact by the operator and environment during the entire amplification and detection reaction.