Apparatus and method for detecting and identifying infectious agents

• US 6,649,356 B2
• Filed: 04/19/2002
• Issued: 11/18/2003
• Est. Priority Date: 12/12/1996
• Status: Expired due to Term

• Current Assignee: Prolume Limited
• Original Assignee: Prolume Limited
• Inventors: Murphy, Randall B., Gaalema, Stephen, Bryan, Bruce J.
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): Padmanabhan, Kartic
• Application Number:
  US10/126,139
• Publication Number:
  US 20030013103A1
• Time in Patent Office: 578 Days
• Field of Search: 435/6, 435/7.1, 435/7.9, 435/288.7, 435/808, 435/973, 435/975, 436/164, 436/172, 436/518, 436/524, 436/527, 436/532, 436/805, 455/57, 455/58, 455/68.1, 455/82.05, 455/82.08, 356/215, 356/222, 356/317
• US Class Current: 506/9
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10T 436/186 Sulfur dioxide

Abstract

Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.

Claims

1. A kit comprising a diagnostic system for detecting infectious agents, comprising:

(a) a microelectronic device comprising;

a substrate;

a plurality of micro-locations defined on the substrate, wherein each micro-location is for linking a macromolecule;

an independent photodetector integrated at each micro-location and optically coupled to each micro-location, each photodetector being configured to generate a sensed signal responsive to the photons of light emitted at the corresponding micro-location when a light-emitting chemical reaction occurs at that micro-location, each photodetector being independent from the photodetectors optically coupled to the other micro-locations; and

an electronic circuit coupled to each photodetector and configured to read the sensed signal generated by each photodector and to generate output data signals therefrom that are indicative of the light emitted at each micro-location by the light-emitting chemical reactions, whereby the device detects photons of light emitted by light-emitting chemical reactions, wherein each micro-location is defined by a portion of the surface of the device;

(b) an anti-ligand;

(c) a first composition comprising a conjugate that comprises a component of a bioluminescence generating system, and an anti-ligand, wherein the anti-ligand specifically binds to an epitope on a surface of the infectious agent; and

(d) a second composition, comprising a second component of the bioluminescence generating system.

2. The kit of claim 1, wherein the component of the bioluminescence generating system is a luciferase or luciferin.

3. The kit of claim 1, wherein:

the compositions comprise a bioluminescence generating system;

the bioluminescence generating system comprises a luciferase and a luciferin.

4. The kit of claim 1, wherein the bioluminescence generating system is selected from the group consisting of those isolated from the ctenophores, coelenterases, mollusca, fish, ostracods, insects, bacteria, a crustacea, annelids, and earthworms.

5. The kit of claim 2, wherein the luciferase is selected from the group consisting of Aequorea, Vargula, Renilla, Obelin, Porichthys, Odontosyllis, Aristostomias, Pachystomias, firefly, and bacterial systems.

6. The kit of claim 1, further comprising a composition comprising a fluorescent protein.

7. The kit of claim 6, wherein the fluorescent protein is selected from the group consisting of green fluorescent protein (GFP), blue fluorescent protein (BFP) and a phycobiliprotein.