×

Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities

  • US 6,649,358 B1
  • Filed: 05/25/2000
  • Issued: 11/18/2003
  • Est. Priority Date: 06/01/1999
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of determining an effect of a compound on a transporter activity of a component, the method comprising:

  • concomitanly flowing through a first channel a first component comprising the transporter activity, a transportable molecule that interacts with said first component, and a second component comprising a receptor to said transportable molecule that competitively binds with the transportable molecule;

    detecting a first signal produced by competitive binding of the receptor to the transportable molecule;

    concomitantly flowing through the first channel the first component, the transportable molecule, the second component, and a third component;

    detecting a second signal produced by the competitive binding of the receptor to the transportable molecule in the presence of the third component; and

    comparing the first and second signals to determine the effects of the third component on the transporter activity of the first component.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×