Color translating UV microscope
First Claim
1. A method of producing an observable image representing the differential absorption of light by a sample, the method comprising:
- a) illuminating the sample with non-visible light;
b) receiving light from the sample as an image of the sample and converting said light image to a visible polychromatic light image; and
c) selecting a color from said polychromatic light image to thereby generate said observable image.
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Accused Products
Abstract
A color translating UV or IR microscope for imaging living or dynamic samples in real time. The microscope includes a UV or IR light source and a first filter for cyclically separating light from the light source into spectral components which illuminate the sample. A device such as an image intensifier converts light received from the sample to visible polychromatic light, e.g., an intensified white light. A second filter set separates the visible polychromatic light into color planes. The first and second filter sets are synchronized with each other so as to enable the color planes to be recombined into a visible multicolor image, wherein each color plane represents illumination of the sample by non-visible light of different wavelengths.
91 Citations
27 Claims
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1. A method of producing an observable image representing the differential absorption of light by a sample, the method comprising:
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a) illuminating the sample with non-visible light;
b) receiving light from the sample as an image of the sample and converting said light image to a visible polychromatic light image; and
c) selecting a color from said polychromatic light image to thereby generate said observable image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of producing an observable image representing the differential absorption of light by a sample, the method comprising:
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a) illuminating the sample with light of a first selected wavelength or wavelength region;
b) receiving light from the sample as an image of the sample and converting said light image to a polychromatic light image; and
c) selecting a pre-determined wavelength or wavelength region from said polychromatic light image to thereby generate said observable image.
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10. A method of producing a visible image representing the differential absorption of light by a sample, the method comprising:
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a) illuminating the sample with non-visible light, the wavelength or wavelength region of the illuminating light being cyclically selected from a pre-determined group of wavelengths or wavelength regions;
b) receiving light from the sample as an image of the sample and converting said light image to a polychromatic light image;
c) filtering said polychromatic light image to thereby obtain a wavelength or wavelength region therefrom for display as said visible image, the displayable light being cyclically selected from a pre-determined group of wavelengths or wavelength regions; and
d) periodically and synchronously changing the wavelengths or wavelength regions of the illuminating light and the displayable light. - View Dependent Claims (11)
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12. An apparatus for producing an observable image representing the differential absorption of light by a sample, the apparatus comprising:
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a) means for illuminating the sample with non-visible light;
b) means for converting light received from the sample as an image thereof to a polychromatic light image; and
c) means for selecting a color from said polychromatic light image to thereby generate said observable image.
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13. A microscope for translating spectral information to a visible color image, comprising:
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a light source providing light substantially not visible to the human eye;
a stage for holding a sample;
a first set of at least two filters for separating light from the light source into spectral components;
a lens system for illuminating the sample with light emanating from the first filter set;
means for converting light received from the sample to visible polychromatic light;
a second set of at least two filters for separating the visible polychromatic light into color planes;
wherein the first and second filter sets are synchronized with each other so as to enable the color planes to be recombined into a visible multicolor image. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
a) the intensity of illuminating spectra from the source;
b) differences in the spectral transmission efficiency of optical components employed in the lens system;
c) differences in the spectral photon to electron conversion efficiency of a photocathode of the image intensifier; and
d) differences in the spectral output of the image intensifier.
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19. A microscope according to claim 13, including an intermediate set of at least one filter disposed so as pass from the sample only the wavelength(s) of the illuminating light.
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20. A microscope according to claim 19, wherein the intermediate filter set is synchronized with the first filter set.
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21. A microscope according to claim 13, including an intermediate set of at least one filter disposed to reject from the sample only the wavelength(s) of the illuminating light, thereby aiding fluorescent or autofluorescent imaging.
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22. A microscope according to claim 21, wherein the intermediate filter set is synchronized with the first and second filter sets.
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23. A microscope according to claim 13, including an intermediate synchronized set of at least one narrowband filter arranged to reject from the sample only the wavelength(s) of the illuminating light in order to enable any Raman re-emission spectra to be viewed.
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24. A microscope according to claim 13, including polarizing means capable of transmitting the wavelength(s) being used for illumination, said polarizing means being located in at least one of first and second positions, the first position being between the light source and the sample, the second position being between the sample and the conversion means.
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25. A microscope according to claim 24, wherein the first and second sets of filters are disposed on wheels mounted to a common shaft, the polarizing means being disposed on a wheel mounted to the common shaft in synchronicity with the first and second filter wheels.
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26. A microscope according to claim 13, including a tubular support means for reducing vibration.
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27. A method of producing an image representing the differential absorption of light by a sample, comprising:
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a) illuminating a sample with light of a first desired wavelength(s) by imposing a first filter between a multi-wavelength source and the sample;
b) receiving light from the sample at a photon gain device which converts the received light to an intensified white light;
c) filtering the intensified white light with a second filter to obtain visible light at a pre-selected wavelength(s) for said desired wavelength;
d) forming an image from said filtered intensified white light; and
e) synchronously changing the first and second filters and repeating steps (a) through (d) to illuminate the sample with light of a second desired wavelength and form an image from visible light obtained from the intensified white light at a second pre-selected wavelength for said second desired wavelength.
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Specification