Method and apparatus for providing high contrast imaging

• US 6,650,916 B2
• Filed: 02/08/2002
• Issued: 11/18/2003
• Est. Priority Date: 11/05/1998
• Status: Expired due to Fees

• Current Assignee: Intellectual Property MVM B.V.
• Original Assignee: Cytoprop, LLC
• Inventors: Meyers, Mark M., Cook, Christopher A.
• Primary Examiner(s): Winakur, Eric F.
• Assistant Examiner(s): Kremer, Matthew J
• Application Number:
  US10/067,783
• Publication Number:
  US 20020111546A1
• Time in Patent Office: 648 Days
• Field of Search: 600/310, 600/322, 600/365, 600/473, 600/330, 600/162, 600/476, 600/309, 600/250, 356/39-42, 356/364, 356/369, 382/128, 382/130, 382/134, 382/274
• US Class Current: 600/322
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14535 for measuring haematocrit
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern

Abstract

An in vivo imaging device having an illumination system that creates a virtual source within a tissue region of a subject in a non-invasive manner. The illumination system transforms a maximum amount of illumination energy from a light source into a high contrast illumination pattern. The illumination pattern is projected onto the object plane in a manner that maximizes the depth to which clear images of sub-surface features can be obtained. The high intensity portion of the illumination pattern is directed onto the object plane outside the field of view of an image capturing device that detects the image. In this configuration, scattered light from within the tissue region interacts with the object being imaged. This illumination technique provides for a high contrast image of sub-surface phenomena such as vein structure, blood flow within veins, gland structure, etc.

Claims

1. An apparatus for detecting optical characteristics of a sub-surface object located in the region of an object plane, comprising:

a light source for providing an illumination beam;

an illumination system that transforms said illumination beam into an illumination pattern having a high intensity portion and a low intensity portion and projects said high intensity portion onto the sub-surface object substantially outside a desired portion of the sub-surface object to be imaged; and

an image capturing device for detecting an image of the desired portion of the sub-surface object, said image being formed by scattered illumination from said illumination pattern that is transmitted through the sub-surface object to said image capturing device,

wherein said illumination system projects said high intensity region outside a field of view of said image capturing device at the object plane and projects said low intensity region within the field of view of said image capturing device.

2. The apparatus of claim 1, further comprising a condenser disposed between said light source and said object plane to collimate said illumination beam.

3. The apparatus of claim 1, said illumination system comprising a folding mirror disposed between said light source and the object plane, said folding mirror including a surface having a substantially reflective outer portion and a substantially transmissive inner portion.

4. The apparatus of claim 1, further comprising an objectived disposed between the object plane and said image capturing device along said image path for magnifying said image onto said image capturing device.

5. The apparatus of claim 4, wherein said illumination system comprises:

an illumination pattern generator disposed between said light source and the object plane to transform said illumination beam into a dual intensity pattern having said high intensity portion and said low intensity portion;

a projection lens disposed between said illumination pattern generator and the object plane that projects said high intensity pattern onto the object plane; and

a folding mirror, disposed between said light source and the object plane and disposed between the object plane and the image capturing device, to direct said projected high intensity illumination portion along said image path onto the object plane.

6. The apparatus of claim 4, wherein said illumination pattern generator comprises an obscuration disposed between said light source and said optical element, said obscuration substantially blocking a first portion of said illumination beam to form said low intensity portion, and permitting substantially unobstructed passage of a second portion of said illumination beam to form said high intensity portion.

7. The apparatus of claim 6, wherein said obscuration is disposed along said illumination path substantially in the plane of a first aperture, wherein an outer diameter of said obscuration corresponds to said field of view of said image capturing device, and wherein said projection lens and said objective images said obscuration onto the object plane.

8. The apparatus claim 5, wherein said illumination pattern generator comprises:

a conical lens disposed between said light source and said projection lens, said conical lens redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

9. The apparatus of claim 8, wherein said conical lens is an axicon.

10. The apparatus of claim 5, wherein said illumination pattern generator comprises a conical grating disposed between said light source and said projection lens, said conical grating redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

11. The apparatus of claim 5, wherein said illumination pattern generator comprises an optical element disposed between said light source and said projection lens, said optical element having a hologram coated onto a surface of said optical element for redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

12. The apparatus of claim 5, further comprising:

a first polarizer disposed between said light source and said folding mirror for polarizing said illumination beam from said light source; and

a second polarizer having a plane of polarization substantially orthogonal to a plane of polarization of said first polarizer and disposed along said image path between the folding mirror and said image capturing device,.

13. A spectral imaging apparatus for non-invasive, in vivo imaging of a subject'"'"'s tissue and blood, comprising:

a light source for illuminating a region of interest;

means for transforming light emanating from said light source into an illumination pattern having a low intensity portion and a high intensity portion;

projection means for projecting said low intensity portion of said illumination pattern onto an object substantially within the region of interest and beneath a surface of the region of interest and for projecting said high intensity portion of said illumination pattern onto the object substantially outside the region of interest; and

an image capturing device for capturing an image of the region of interest formed by scattered illumination from said high intensity illumination portion that is transmitted through the object and the region of interest to said image capturing device.

14. The apparatus of claim 13, wherein said projection means directs said high intensity portion of said illumination pattern to a portion of said region of interest outside a field of view of said image capturing means.

15. The apparatus of claim 14, wherein light from said high intensity portion of said illumination pattern interacts with matter within said region of interest and is scattered by one or more scattering events, thereby forming a sub-surface illumination source to illuminate said object.

16. The apparatus of claim 15, wherein said image is formed by a substantial portion of said sub-surface illumination source being transmitted through said object along said image path.

21. The method of claim 15, wherein step (b) further comprises:

providing an optical element to redistribute said light into an annular pattern which corresponds to said illumination pattern.

27. The apparatus of claim 16, wherein said conical lens is an axicon.

17. A method for creating a source of illumination in a sub-surface region of an object that contains an area of interest, wherein the area of interest is illuminated from all directions about an object plane wherein the object is located, wherein an image of the area of interest is detected by an image capturing device, comprising the steps of:

(a) transforming light from a light source into an illumination pattern having a high intensity portion and a low intensity portion;

(b) directing said illumination pattern onto a surface of the object such that said high intensity portion of said illumination pattern is incident upon the object plane substantially outside a field of view of the image capturing device and said low intensity portion is incident upon the object plane substantially within the field of view of the image capturing device; and

(d) detecting scattered light that interacts with the area of interest with the image capturing device, wherein said high intensity portion of said illumination pattern undergoes one or more scattering events in the sub-surface region.

18. The method of claim 17, further comprising the step of:

(e) performing a transmission measurement of said sub-surface region.

19. The method of claim 18, wherein step (b) further comprises:

providing a folding mirror having a first surface that includes a substantially reflective outer portion and a substantially transmissive inner portion, wherein light reflected off said substantially reflective outer portion forms said high intensity portion of said illumination pattern.

20. The method of claim 17, wherein step (b) further comprises:

blocking a portion of said light from said source to form said low intensity portion of said illumination pattern.

22. A spectral imaging apparatus for non-invasive, in vivo imaging of mammalian tissue and blood in a living body, comprising:

a light source for providing an illumination beam;

an illumination system that transforms said illumination beam into an illumination pattern having a high intensity portion and a low intensity portion and projects said illumination pattern below the surface of the tissue; and

an image capturing device for detecting an image of the desired portion of the sub-surface region, said image being formed by scattered illumination from said illumination pattern that is transmitted through the sub-surface region to said image capturing device,

wherein said illumination system projects said high intensity portion substantially outside a field of view of said image capturing device at the surface of the tissue and projects said low intensity portion substantially within the field of view of said image capturing device.

23. The apparatus of claim 22, wherein said illumination system comprises:

an illumination pattern generator disposed between said light source and the surface of the tissue to transform said illumination beam into a dual intensity pattern having said high intensity portion and said low intensity portion;

a projection lens disposed between said illumination pattern generator and the surface of the tissue that projects said high intensity portion toward the surface of the tissue; and

a folding mirror, disposed between said light source and the surface of the tissue and disposed between the surface of the tissue and the image capturing device, to direct said projected high intensity illumination portion onto the surface of the tissue and to direct scattered light from the subsurface region of the tissue to said image capturing device.

24. The apparatus of claim 23, wherein said illumination pattern generator comprises an obscuration disposed between said light source and said optical element, said obscuration substantially blocking a first portion of said illumination beam to form said low intensity portion, and permitting substantially unobstructed passage of a second portion of said illumination beam to form said high intensity portion.

25. The apparatus of claim 24, wherein said obscuration is disposed along said illumination path substantially in the plane of a first aperture, wherein an outer diameter of said obscuration corresponds to said field of view of said image capturing device, and wherein said projection lens and said objective images said obscuration onto the object plane.

26. The apparatus of claim 23, wherein said illumination pattern generator comprises:

a conical lens disposed between said light source and said projection lens, said conical lens redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

28. The apparatus of claim 23, wherein said illumination pattern generator comprises a conical grating disposed between said light source and said projection lens, said conical grating redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

29. The apparatus of claim 23, wherein said illumination pattern generator comprises an optical element disposed between said light source and said projection lens, said optical element having a hologram coated onto a surface of said optical element for redistributing said illumination beam into an annular pattern projected onto the object plane by said projection lens, wherein said annular pattern has a low intensity central portion and a high intensity outer portion.

30. The apparatus of claim 23, further comprising:

a first polarizer disposed between said light source and said folding mirror for polarizing said illumination beam from said light source; and

a second polarizer having a plane of polarization substantially orthogonal to a plane of polarization of said first polarizer and disposed along said image path between the folding mirror and said image capturing device.