Method for the express diagnosis of the physiological condition of a biological object and device for realizing the same

• US 6,650,933 B1
• Filed: 10/25/2001
• Issued: 11/18/2003
• Est. Priority Date: 04/28/1999
• Status: Expired due to Fees

• Current Assignee: Anatoly Ivanovich Obabkov
• Original Assignee: Anatoly Ivanovich Obabkov
• Inventors: Obabkov, Anatoly Ivanovich
• Primary Examiner(s): Hindenburg, Max F.
• Assistant Examiner(s): SZMAL, BRIAN SCOTT
• Application Number:
  US10/019,361
• Time in Patent Office: 754 Days
• Field of Search: 600/547, 600/300, 600/549, 600/551, 600/573, 436/65, 436/164, 436/166
• US Class Current: 600/547
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence

Abstract

The present invention relates to a method that involves collecting a sample of biological liquid, placing the same in a cell (1) for analysis, supplying a current through the cell from a frequency- and amplitude-adjustable generator (2) and measuring the conductivity Y1. The method also involves using a biological-liquid reference (12) through which a current is supplied at the same amplitude and frequency before measuring its conductivity Yr. The method further involves calculating a value N₁=Y1/Yr and the biological object is in a normal condition when N₁<1, whereas a potential disease is diagnosed when N₁>1. The device of the present invention comprises an analysis cell (1) having a generator (2) connected thereto and also includes a differential amplifier (3), a detector (4) and a measuring unit (5) connected in series. The differential amplifier (3) includes first and second current summing inputs (6, 7) and the generator (2) is connected to the first summing input (6) of said differential amplifier (3). An amplifier (8) is also n provided and has first and second current summing inputs (9, 10), while an electrode (13) is connected to the second summing input (7) of the differential amplifier (3) and is capable of three-dimensional displacement so as to be brought into contact with the biological liquid in the analysis cell (1) and with the reference (12). The detector (4) is connected to the measuring unit (5) through the first summing input (9) of the added amplifier (8), while a voltage regulator (11) is connected thereto through the second summing input (10) of said amplifier (8).

Claims

1. A method of diagnosis of the physiological condition of a biological object, which involves collecting a biological fluid sample from a biological object, placing the sample in an analysis cell (1), passing an alternating current through the cell (1) for the analysis with a biological liquid, and measuring the biological fluid sample conductivity Y1 used for assessment of the physiological condition of the biological object, wherein the biological fluid sample is collected from the biological object after awakening, a reference (12) biological fluid is additionally used, an alternating current of the same frequency and amplitude is passed through the reference biological fluid (12) and its conductivity Y_R is measured at constant temperatures of the reference and the analysis cell (1) containing the biological fluid, the conductivity values Y1 and Y_R are compared by determining the ratio N₁=Y1/Y_R, where N₁ lower than 1 evidences the normal condition of the biological object and N₁ greater than 1 attests to a disorder in the physiological condition of the biological object.

2. The method according to claim 1, wherein the biological fluid sample collected from a reference biological object and used as the biological fluid reference (12) is placed in the reference cell is similar as to its electrical parameters to the analysis cell, and the conductivity Y_R of the biological fluid sample contained in the reference cell is measured.

3. The method according to claim 1, wherein a resistor is used as the biological fluid reference (12), which has the conductivity equal to that of the biological fluid and the analysis cell.

4. The method according to claim 1, wherein a second biological fluid sample is collected from the biological object before a first meal and is placed in an additional analysis cell (18) similar as to its electrical parameters to the said analysis cell (1), the conductivity Y2 of the second sample is measured, the ratio N₂=Y2/Y_R is determined by the biological fluid conductivity in the additional analysis cell (18) and that in the reference cell, and the condition of the immune system is assessed by the compliance with the relation N₁/N₂>

1, where at the value N₁<

1 the immune system activity grows, but if N₁ approaches to 1 it is reduced.

5. The method according to claim 1, wherein saliva is used as the biological fluid.

6. The method according to claim 1, wherein urea is used as the biological fluid.

7. The method according to claim 1, wherein blood is used as the biological fluid.

8. A device for diagnosis of the physiological condition of a biological object comprising

an analysis cell (1) intended for placing a biological fluid therein,

an alternating current generator (2) connected to the cell (1), and

a differential amplifier (3), a detector (4), a measuring unit (5) that are connected in series,

the cell (1) being made so as to allow its connection to the differential amplifier (3), wherein the differential amplifier (3) is made with first (6) and second (7) current summing inputs and providing summation on the mentioned current summing inputs (6), (7), the alternating current generator (2) is connected to the first summing input (6) of the differential amplifier;

further comprising an amplifier (8), which is made with first and the second summing inputs (9), (10) and providing summation on the mentioned inputs (9), (10),

a voltage regulator (11),

a biological fluid reference (12),

an electrode (13) connected to the second summing input (7) of the differential amplifier (3) and made so as to allow spatial displacement of the electrode for making alternate contact with the analysis cell (1) for analysis or with the biological fluid reference (12),

the detector (4) being connected to the measuring unit (5) through the first input (9) of the amplifier (8) and the voltage regulator (11) being connected thereto through the second input (10) of the amplifier (8).

9. The device according to claim 8, further comprising a correspondent to the electrode (13), which is made in the form of a resistor (14) having the conductivity equal to that of the electrode (13) and connected to the second input (7) of the differential amplifier (3) through a switch (15).

10. The device according to claim 8, wherein the generator (2) is made capable of modulating the output signal frequency and amplitude.

11. The device according to claim 8, wherein the electrode (13) is made in the form of a coaxial probe with the cone-shaped tip.