Methods for encoding combinatorial libraries

• US 6,653,153 B2
• Filed: 07/03/2001
• Issued: 11/25/2003
• Est. Priority Date: 07/03/2000
• Status: Expired due to Fees

• Current Assignee: Merck & Co., Inc.
• Original Assignee: Merck & Co., Inc.
• Inventors: Xiong, Yusheng, Chapman, Kevin, Wang, Tiebang
• Primary Examiner(s): Ponnaluri, Padmashri
• Assistant Examiner(s): Tran, My Chau T.
• Application Number:
  US09/898,142
• Publication Number:
  US 20020022237A1
• Time in Patent Office: 875 Days
• Field of Search: 435/4, 435/174, 435/180, 436/518, 436/84, 436/85, 436/100, 436/102, 436/120, 436/528, 436/531, 546/207, 546/208
• US Class Current: 506/31
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00572 Chemical means
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C40B 50/16 involving encoding steps
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10T 436/182 Organic or sulfhydryl containing [e.g., mercaptan, hydrogen, sulfide, etc.]

Abstract

A combinatorial library comprises a plurality of ligand-bearing support units, where the ligand-bearing support units comprise (a) a solid carrier, (b) one or more ligands covalently bound to the solid carrier, and (c) one or more encoding metal salts impregnated on the support unit, where the distribution of the cations of the encoding metal salts provides a code for identifying the ligand or ligands that are attached to the support units, and the cations have been stabilized against dissolution by treatment with one or more anions that form insoluble or poorly soluble salts of the cations.

Claims

1. A method of preparing an encoded combinatorial library comprising a plurality of ligand-bearing support units, wherein said ligand-bearing support units comprise (a) a solid carrier, (b) one or more ligands covalently bound to said solid carrier, and (c) a plurality of encoding metal salts impregnated on said support unit, said metal salts providing a code for identifying said ligand, comprising the steps of:

(1) providing support units comprising a solid carrier having functional groups, said functional groups being optionally connected to linker groups, said linker groups being organic residues covalently bound to the functional groups of said solid carrier and having functional groups for covalent binding to said ligand;

(2) covalently attaching a ligand or a first sub-unit of a ligand that will have more than one sub-unit to the functional group of said carrier or to the functional group of an optional linker group, said sub-unit having a functional group for covalent binding to a second sub-unit;

(3) impregnating said support unit with one or more encoding metal salts, said salts being comprised of one or more encoding metal cations, said encoding metal cations being distributed in their natural isotope abundance or in a non-natural isotope abundance, wherein the encoding metal cations provide a code for identifying the ligand or sub-unit that is attached to said support unit, wherein the encoding metal salts are impregnated onto the support unit by treating the support unit with a solution of a soluble salt of the encoding metal cations to form a support unit comprising the soluble encoding metal cations, and then treating said support unit comprising said soluble encoding metal salts with a solution of a salt having an anion that forms insoluble or poorly soluble salts when combined with said encoding metal salts, thereby yielding a stabilized encoding metal salt, said salt being stabilized against dissolution from said support unit;

wherein step (3) is optionally carried out before or after step (2) or concurrently with step (2).

2. A method of preparing an encoded combinatorial library comprising a plurality of ligand-bearing support units, as recited in claim 1, wherein said ligands comprise two or more sub-units, comprising the further steps of:

(1) covalently attaching a second sub-unit to the functional group of said first sub-unit, wherein said second sub-unit is optionally the same as the first sub-unit or different, wherein said second sub-unit optionally has a functional group that optionally may be used for adding a third sub-unit;

(2) impregnating said support unit with one or more encoding metal salts, said salts being comprised of one or more encoding metal cations, said encoding metal cations being distributed in their natural isotope abundance or in a non-natural isotope abundance, wherein said encoding metal cations provide a code for identifying said second sub-unit, wherein the encoding metal salts are impregnated onto the support unit by treating the support unit with a solution of a soluble salt of the encoding metal cation to form a support unit comprising a soluble encoding metal salt, and then treating said support unit comprising said soluble encoding metal salt with a solution of a salt having an anion that forms insoluble or poorly soluble salts when combined with said encoding metal cations, thereby yielding a stabilized encoding metal salt, said salt being stabilized against dissolution from said support unit; and

(3) optionally repeating said steps (1) and (2) one or more times to add additional sub-units to form a ligand comprising a plurality of sub-units, said ligand being identifiable by measurement of the distribution of metal cations on said support unit;

wherein step (1) is optionally carried out before or after step (2) or concurrently with step (2) in each repetition of steps (1) and (2).

10. The method as recited in claim 1, wherein said library is made by a split-pool synthesis.

3. A method of preparing an encoded combinatorial library comprising a plurality of ligand-bearing support units, wherein said ligand-bearing support units comprise (a) a solid carrier, (b) one or more ligands covalently bound to said solid carrier, wherein said ligands are comprised of two or more sub-units, and (c) a plurality of encoding metal salts impregnated on said support unit, said metal salts providing a code for identifying said ligand, comprising the steps of:

(1) providing support units comprising a solid carrier having functional groups, said functional groups being optionally connected to linker groups, said linker groups being organic residues covalently bound to the functional groups of said solid carrier and having functional groups for covalent bonding to said ligand;

(2) covalently attaching a first sub-unit to the functional group of said carrier or to the functional group of said optional linker group, said sub-unit having a functional group for covalent bonding to a second sub-unit;

(3) impregnating said support unit with one or more encoding metal salts, said salts being comprised of one or more encoding metal cations, said encoding metal cations being distributed in their natural isotope abundance or in a non-natural isotope abundance, wherein the combination of encoding metal cations provides a code for identifying the sub-unit that is attached to said support unit, wherein the encoding metal salts are impregnated onto the support unit by treating the support unit with a solution of a soluble salt of the encoding metal cation to form a support unit comprising the soluble encoding metal salt, and then treating said support unit comprising said soluble encoding metal salt with a solution of a salt having an anion that forms insoluble or poorly soluble salts when combined with said encoding metal cations, thereby yielding an encoding metal salt that is stabilized against dissolution from said support unit;

(4) covalently attaching a second sub-unit to the functional group of said first sub-unit, wherein said second sub-unit may be the same as the first sub-unit or different, wherein said second sub-unit optionally has a functional group that optionally may be used for adding a third sub-unit;

(5) impregnating said support unit with one or more encoding metal salts, said salts being comprised of one or more encoding metal cations, said encoding metal cations being distributed in their natural isotope abundance or in a non-natural isotope abundance, wherein said encoding metal cations provide a code for identifying said second sub-unit, wherein the encoding metal salts are impregnated onto the support unit by treating the support unit with a solution of a soluble salt of the encoding metal cation to form a support unit comprising a soluble encoding metal salt, and then treating said support unit comprising said soluble encoding metal salt with a solution of a salt having an anion that forms insoluble or poorly soluble salts when combined with said encoding metal cations, thereby yielding an encoding metal salt that is stabilized against dissolution from said support unit;

(6) repeating said steps (4) and (5) one or more times to add additional sub-units to form a ligand comprising a plurality of sub-units, said ligand being identifiable by measurement of the distribution of metal cations on said support unit;

wherein step (2) is optionally carried out before or after step (3) or concurrently with step (3), step (4) is optionally carried out before or after step (5) or concurrently with step (5), and in subsequent repetitions of steps (4) and (5) as recited in step (6), step (4) is optionally carried out before or after step (5) or concurrently with step (5).

4. The method as recited in claim 3, wherein said solid carrier is a synthetic polymeric compound, and said support units are porous beads.

5. The method as recited in claim 4, wherein said polymeric compound is polystyrene, optionally crosslinked with divinylbenzene, wherein said polystyrene comprises functional groups, and said ligands or said optional linker groups are covalently bound to said functional groups of said polystyrene.

6. The method as recited in claim 3, wherein said encoding metal cations are selected from the group consisting of the transition metals, the lanthanides, the actinides, Sr, Ba, Tl, In, Sb, and Bi.

7. The method as recited in claim 5, wherein said encoding metal cations are selected from the group consisting of the Group VIIIB, IB and IIB transition metals.

8. The method as recited in claim 3, wherein said encoding metal cations are selected from the group consisting of Pd, Ru, Rh, Pt, Ag, Ni, Cu, Co, Hg, and individual isotopes thereof.

9. The method as recited in claim 3, wherein said anion is sulfide.

17. The method as recited in claim 4, wherein said polymeric compound is aminomethyl functionalized polystyrene.

18. The method as recited in claim 17, wherein said optional linker group is HMBA.

11. In a method of preparing a combinatorial library comprising a plurality of support units, wherein each support unit comprises (a) a solid carrier, (b) one or more ligands covalently bound to said solid carrier or to a linker group that is covalently bound to said solid carrier, and (c) one or more encoding metal salts impregnated on said support unit, wherein said encoding metal salts comprise encoding metal cations, the distribution of said cations providing a code that identifies said ligand or ligands, the improvement that said encoding metal cations are stabilized against dissolution by treatment of said support units with a solution that comprises one or more anions that form insoluble or poorly soluble salts of said encoding metal cations in the solvent or solvents which are used to prepare said combinatorial library, thereby yielding an encoding metal salt that is stabilized against dissolution.

12. The improvement as recited in claim 11, wherein said encoding metal cations form soluble salts with one or more anions selected from the group consisting of nitrates, hydroxides, chlorides, acetates, and sulfates, and said encoding metal cations form insoluble or poorly soluble salts with one or more anionic groups selected from the group consisting of sulfides, sulfates, oxides, hydroxides, halides and carbonates.

13. The improvement as recited in claim 11, wherein said encoding metal cations are selected from the group consisting of the transition metals, the lanthanides, the actinides, Sr, Ba, Tl, In, Sb, and Bi.

14. The improvement as recited in claim 11, wherein said anions are sulfide anions.

15. The improvement as recited in claim 11, wherein said encoding metal cations are selected from the group consisting of the Group VIIIB, IB and IIB transition metals.

16. The improvement as recited in claim 11, wherein said encoding metal cations are selected from the group consisting of Pd, Ru, Rh, Pt, Ag, Ni, Cu, Co, Hg, and individual isotopes thereof.

19. The method as recited in claim 11, wherein said solid carrier is aminomethyl functionalized polystyrene.

20. The method as recited in claim 19, wherein said linker group is HMBA.