Nucleotide compounds including a rigid linker
First Claim
1. A nucleoside/tide compound comprising the structure:
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wherein;
NUC is a nucleoside/tide having a nucleobase B;
L is a rigid linkage having the structure;
wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2; and
each of W, X, Y and Z is independently selected from CH and N, including substituted forms thereof;
S is a spacer selected from C1-C8 lower alkyl, lower alkylene oxide, amide, carbamate, and sulfonamide;
wherein lower alkylene oxide is selected from the structures;
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Accused Products
Abstract
A nucleoside/tide compound having the structure:
is described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure:
wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nucleoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
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Citations
37 Claims
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1. A nucleoside/tide compound comprising the structure:
-
wherein; NUC is a nucleoside/tide having a nucleobase B;
L is a rigid linkage having the structure;
wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2; and
each of W, X, Y and Z is independently selected from CH and N, including substituted forms thereof;
S is a spacer selected from C1-C8 lower alkyl, lower alkylene oxide, amide, carbamate, and sulfonamide;
wherein lower alkylene oxide is selected from the structures;
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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Specification