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Fluorescent quenching detection reagents and methods

  • US 6,653,473 B2
  • Filed: 02/26/2002
  • Issued: 11/25/2003
  • Est. Priority Date: 12/08/1999
  • Status: Expired due to Term
First Claim
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1. A resorufin phosphoramidite reagent having the formula:

  • embedded imagewherein,Z is O or S;

    each of R21, R22, R23, R24, R25, R26 and R27 is independently selected from H, halogen, NO2, SO3R, SO2N(R)2, C(O)OR, C(O)N(R)2, CN, CNS, OR, OC(O)R, SR, CF3, NHC(O)R, N(R)2, N[R]3 and —

    L—

    X;

    wherein each R is independently H, (C1-C8)alkyl, aryl or heteroaryl, or a blocking group compatible with oligonucleotide synthesis;



    L—

    X is a linker of from 1 to 20 atoms, that is acyclic, cyclic or a combination thereof, having main chain atoms selected from C, N, O, S, Si and P; and

    X is a phosphoramidite;

    with the proviso that at least one of R21, R22, R23, R24, R25, R26, and R27 is —

    L—

    X.

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