Tray for macromolecule crystallization and method of using the same

• US 6,656,267 B2
• Filed: 07/10/2001
• Issued: 12/02/2003
• Est. Priority Date: 07/10/2001
• Status: Expired due to Fees

• Current Assignee: Structural Genomix, Inc. (Eli Lilly and Company)
• Original Assignee: Structural Genomix, Inc. (Eli Lilly and Company)
• Inventors: Newman, Janet
• Primary Examiner(s): HITESHEW, FELISA CARLA
• Application Number:
  US09/903,341
• Publication Number:
  US 20030010278A1
• Time in Patent Office: 875 Days
• Field of Search: 117/1, 117/5, 117/11, 117/206, 117/925, 117/927, 422/245.1
• US Class Current: 117/11
• CPC Class Codes: B01L 3/06 Crystallising dishes
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C30B 29/58 Macromolecular compounds
• CPC Class Codes: C30B 7/00 Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00
• CPC Class Codes: Y10T 117/1024 for crystallization from liquid or supercritical state

Abstract

A crystallization tray includes a plurality of crystallization cells, each cell having a reservoir adapted to receive an equilibrating solution, a shelf located adjacent to the reservoir and adapted for use as a temporary cryogenic holding area for a crystallized substance and/or a sample holding area, and a sample drop receptacle carried by the shelf and adapted to receive a sample drop including a crystallizable substance. A related method for forming macromolecular crystals includes dispensing an equilibrating solution in the reservoirs, dispensing a plurality of macromolecular solution droplets in the sample drop receptacles, covering the cells with a cover; and crystallizing the crystallizable substance by vapor diffusion.

Claims

1. A method of forming macromolecular crystals, comprising the steps of:

providing a macromolecule crystallization tray including a plurality of crystallization cells, each cell including a reservoir adapted to receive a equilibrating solution, a shelf located adjacent to the reservoir and adapted for use as at least one of a sample drop holding area and a temporary cryogenic holding area for a crystallized substance, and a sample drop receptacle within the shelf and adapted to receive a sample drop including a crystallizable substance;

dispensing a equilibrating solution in the reservoirs;

dispensing a plurality of macromolecular solution droplets in the sample drop receptacles;

coveting the cells with a cover; and

crystallizing the crystallizable substance by vapor diffusion.

2. The method of claim 1, further including moving the crystallized substance from the sample drop receptacle to the shelf, and contacting the crystallized substance with a cryoprotectant.

3. The method of claim 2, wherein the shelf is flat, includes opposite ends, and a majority shelf portion, the sample drop receptacle is located adjacent one of the ends, the majority shelf portion is located between the sample drop receptacle and the opposite end, and the step of moving the crystallized substance includes moving the crystallized substance from the sample drop receptacle to the majority shelf portion of the shelf.

4. The method of claim 1, wherein the sample drop receptacle includes a flat bottom that is optically clear.

5. The method of claim 1, wherein the tray includes a top wall, a ridge extending upwardly from the top wall and delineating each cell, the ridge adapted to support the cover for isolating each cell from ambient and from each other, the method further including using a blade between ridges of adjacent cells to vat the cover around one or more cells, and removing the cover around one or more cells.

6. The method of claim 5, wherein the cells and ridges are rectangular.

7. The method of claim 5, wherein the ridges define spaces between the ridges of adjacent cells, and a width of the ridges is greater than a width of the spaces.

8. The method of claim 1, wherein the tray includes four corners, only one of the corners having a unique configuration to mark the location of a first cell of the plurality of cells and a standard orientation for the tray, and the method further including orienting the tray so that the corner having a unique configuration is in an upper left hand corner of the tray.

9. The method of claim 1, wherein the tray includes ninety-six cells.

10. The method of claim 1, wherein the tray is rectangular.

11. The meted of claim 1, further including stacking the tray on top of another tray.

12. The method of claim 1, wherein the tray includes a rectangular base the carries the plurality of cells, a rectangular skirt surrounding the base, and a step connecting the skirt to the base.

13. The method of claim 1, wherein the shelf includes a first end located closer to the user and an opposite second end located farther away from the user when the tray is in front of the user and the user is looking down at the tray, the shelf further includes a majority shelf portion, the sample drop receptacle is located adjacent the first end, and the majority shelf portion is located between the sample drop receptacle and the opposite second end, aid the method further includes moving the crystallized substance from the sample drop receptacle forward to the majority shelf portion.

14. The method of claim 1, wherein each cell includes a left side and a right side when the tray is in front of the user and the user is looking down at the tray, and each shelf is located on the left side of the cell.

15. The method of claim 1, wherein the tray includes a top wall and three knobs located adjacent three respective corners of the tray, and the method further including orienting the tray using the three knobs.

16. The method of claim 1, wherein the reservoir includes a reservoir volume and the receptacle includes a receptacle volume, and the ratio of the reservoir volume to the receptacle volume is at least about 40:

1.

17. The method of claim 1, wherein the reservoir includes a reservoir volume and the receptacle includes a receptacle volume, and the ratio of the reservoir volume to the receptacle volume is at least about 50:

1.

18. The method of claim 1, wherein the reservoir includes a reservoir volume and the receptacle includes a receptacle volume, and the ratio of the reservoir volume to the receptacle volume is at least about 75:

1.

19. The method of claim 1, wherein the reservoir includes a reservoir volume and the receptacle includes a receptacle volume, and the ratio of the reservoir volume to the receptacle volume is at least about 100:

1.

20. The method of claim 1, wherein the reservoir includes a reservoir volume and the receptacle includes a receptacle volume, and the ratio of the reservoir volume to the receptacle volume is sufficient to hold a protein sample and equilibration solution volumes sufficient to drive crystallization of a crystallizable protein to completion.

21. A method of forming macromolecular crystals, comprising the steps of:

providing a macromolecule crystallization tray including four corners and a plurality of rectangular crystallization cells, each cell including a reservoir adapted to receive a equilibrating solution, a shelf located adjacent to the reservoir, at least one sample drop area carried by the shelf to receive a sample drop including a crystallizable substance, each cell including a left side and a right side when the tray is in front of the user and the user is looking down at the tray, each shelf located on the left side of the cell, only one of the four corners having a unique configuration to mark the location of a first cell of the plurality of cells and a standard orientation for the tray, the tray further including a top wall, a ridge extending upwardly from the top wall and delineating each cell, the ridge adapted to support a cover spaced from and parallel to the top wall for isolating each cell from ambient and from each other,

orienting the tray so that the corner having a unique configuration is in an upper left hand corner of the tray;

dispensing a equilibrating solution in the reservoirs;

dispensing a plurality of macromolecular solution droplets in the sample drop areas;

covering the cells with a cover so that the cover is spaced from and parallel to the top wall and isolates each cell from ambient and from each other; and

crystallizing the crystallizable substance by vapor diffusion.

22. A method of forming macromolecular crystals, comprising the steps of:

providing a macromolecule crystallization tray including a plurality of crystallization cells, each cell including a reservoir adapted to receive a equilibrating solution, a shelf located adjacent to the reservoir, at least one sample drop area carried by the shelf to receive a sample drop including a crystallizable substance, each cell including a left side and a right side when the tray is in front of the user and the user is looking down at the tray, each shelf located on the left side of the cell, the fray further including a top wall, a ridge extending upwardly from the top wall and delineating each cell, the ridge adapted to support a cover spaced from and parallel to the top wall for isolating each cell from ambient and from each other,

dispensing a equilibrating solution in the reservoirs;

dispensing a plurality of macromolecular solution droplets in the sample drop areas;

covering the cells with a cover so that the cover is spaced from and parallel to the top wall and isolates each cell from ambient and from each other; and

crystallizing the crystallizable substance by vapor diffusion.