Apparatus for automatic implementation of chemical or biological methods

• US 6,656,724 B1
• Filed: 05/17/2000
• Issued: 12/02/2003
• Est. Priority Date: 11/19/1997
• Status: Expired due to Term

• Current Assignee: F Hoffmann-La Roche AG (Roche Holding AG)
• Original Assignee: MWG-Biotech AG (Eurofins Scientific SE)
• Inventors: Greber, Martin, Kutsch, Peter, Heimberg, Wolfgang, Weichselgartner, Michael
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US09/554,743
• Time in Patent Office: 1,294 Days
• Field of Search: 435/286.1, 435/286.4, 435/286.5, 435/287.2, 435/288.3, 435/303.1, 435/809, 435/287.3, 422/99, 422/100, 422/104, 422/65, 422/63
• US Class Current: 435/286.4
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

The invention relates to a device for automatic implementation of chemical or biological methods in sample vessels, more particularly for sequencing and amplifying nucleic acid sequences, including a pipette apparatus and a thermocycler, the pipette apparatus comprising a pipette arm for pipetting sample substances and/or chemicals. The invention is characterized in that the pipette apparatus and the thermocycler are configured as spatially separated workstations and that a handling arm for moving at least one sample vessel containing sample substances or chemicals and configured separate from the workstations is provided.

Claims

1. A device for implementing a chemical or biological method in a sample vessel supported by a pipette plate, and for sequencing and amplifying nucleic acid sequences, comprising:

a thermocycler disposed on a single worksurface, said thermocycler being operable to receive said pipette plate, and to affect the temperature of said sample vessel;

a pipette station that is disposed on said worksurface and is spatially separated from said thermocycler; and

a robotic arm having a pipette arm and a handling device, said pipette arm being operable to pipette a sample substance into said sample vessel, and said handling device being operable to grip and release the pipette plate and to transport said pipette plate to and from said pipette station and said thermocycler.

2. The device as defined in claim 1, wherein said pipette arm has a lower end, configured to receive a disposable pipette tip, and said pipette arm communicates said pipette tip with a plurality of pumps via corresponding tubes, said plurality of pumps includes a fine metering pump and a wash pump, the wash pump having a throughput higher than a throughput of said fine metering pump for flushing said pipette tip, and said device further comprising a pipette tip magazine for supplying said disposable pipette tips and a pipette tip sweeper for sweeping said disposable pipette tips, both said magazine and said sweeper being disposed on said worksurface, and said magazine being operable to supply said disposable pipette tip for connection to said lower end of said pipette arm, and said sweeper being operable to sweep and dispose of said disposable pipette tip after use.

3. The device as defined in claim 2, further comprising an electrically-conductive, freely-protruding pointer and a sensor, said pointer and said sensor together being mounted on said pipette tip magazine and being operable to check whether said pipette tip is properly arranged on said pipette arm.

5. The device as defined in claim 1, wherein said sample vessel includes a cover, and said pipette arm comprises a cover actuating arm that is operative to open and to close said sample vessels cover.

6. A device for implementing a chemical or biological method in a sample vessel that is supported by a pipette plate and for sequencing and amplifying nucleic acid sequences, comprising:

a thermocycler disposed on a single worksurface, said thermocycler being operable to receive said pipette plate, and to affect the temperature of said sample vessel;

a pipette station that is disposed on said worksurface and is spatially separated from said thermocycler; and

a handling system comprising a single rail, which is disposed adjacent to said worksurface and extends generally parallel with a plane defined by said worksurface, and a pipette arm and a handling device supported by said rail,

said pipette arm being operable to pipette a sample substance into said sample vessel, and said handling device being operable to grip and release the pipette plate, said pipette arm and said handling device being further operable to move along said rail, whereby said sample vessel is filled with said sample material and transported in said pipette plate to and from said pipette station and said thermocycler by said handling system.

4. The device as defined in claim 11, further comprising a multitasking controller that is operative to control said pipette arm, said handling device and said thermocycler, whereby said controller controls said pipette arm to pipette material into said sampling vessel, said handling device to grip or release said pipette plate and transport said pipette plate to and from predetermined locations on the worksurface, and said thermocycler to subject said sample vessel to a predetermine temperature profile.

7. The device as defined in claim 6, wherein said pipette arm has a lower end configured to receive a disposable pipette tip, and said pipette arm communicates said pipette tip with a plurality of pumps via corresponding tubes, said plurality of pumps includes a fine metering pump and a wash pump, the wash pump having a throughput higher than a throughput of said fine metering pump for flushing said pipette tip.

8. The device as defined in claim 7, further comprising a pipette tip magazine for supplying said disposable pipette tip and a pipette tip sweeper for sweeping said disposable pipette tip, both said magazine and said sweeper being disposed on said worksurface, and said magazine being operable to supply said disposable pipette tip for connection to said lower end of said pipette arm, and said sweeper being operable to sweep and dispose of said disposable pipette tip after use.

9. The device as defined in claim 8, further comprising an electrically-conductive, freely-protruding pointer and a sensor, said pointer and said sensor together being mounted on said pipette tip magazine and being operable to check whether said pipette tip is properly arranged on said pipette arm lower end.

10. The device as defined in claim 6, wherein said sample vessel includes a cover, and said pipette arm comprises a cover actuating arm that is operative to open and to close said sample vessel cover.

11. The device as defined in claim 6, further comprising a multitasking controller that is operative to control said pipette arm, said handling device and said thermocycler, whereby said controller controls said pipette arm to pipette material into said sampling vessel, said handling device to grip or release said pipette plate and transport said pipette plate to and from predetermined locations on the worksurface, and said thermocycler to subject said sample vessel to a predetermine temperature profile.