Plant expression constructs
First Claim
Patent Images
1. A DNA construct comprising a chimeric promoter DNA sequence comprising SEQ ID NO:
- 28;
a structural DNA sequence; and
a 3′
non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′
end of the RNA sequence;
wherein the structural DNA sequence is operably linked to the chimeric promoter and the 3′
non-translated region, and the chimeric promoter DNA sequence is heterologous with respect to the structural DNA sequence.
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Accused Products
Abstract
The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.
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Citations
6 Claims
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1. A DNA construct comprising a chimeric promoter DNA sequence comprising SEQ ID NO:
- 28;
a structural DNA sequence; and
a 3′
non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′
end of the RNA sequence;
wherein the structural DNA sequence is operably linked to the chimeric promoter and the 3′
non-translated region, and the chimeric promoter DNA sequence is heterologous with respect to the structural DNA sequence. - View Dependent Claims (2, 3, 4)
- 28;
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5. A method of expressing a structural DNA sequence in a plant, the method comprising:
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(1) providing a DNA construct comprising a promoter that is functional in a plant cell, the promoter comprising SEQ ID NO;
28;
a structural DNA sequence; and
a 3′
non-translated region that functions to cause the addition of polyadenylated nucleotides to the 3′
end of the RNA sequence;
wherein the structural DNA sequence is operably linked to the promoter and the 3′
non-translated region, and the promoter is heterologous with respect to the structural DNA sequence;
(2) introducing the DNA construct into a plant cell; and
(3) regenerating the plant cell to produce the plant such that the structural DNA sequence is expressible in the plant.
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6. A DNA construct comprising a chimeric promoter DNA sequence comprising SEQ ID NO:
- 28;
an aroA;
CP4 structural DNA sequence; and
a 3′
non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′
end of the RNA sequence;
wherein the structural DNA sequence is operably linked to the chimeric promoter and the 3′
non-translated region, and the chimeric promoter DNA sequence is heterologous with respect to the structural DNA sequence.
- 28;
Specification
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Current AssigneeMonsanto Technology LLC (Bayer AG)
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Original AssigneeMonsanto Technology LLC (Bayer AG)
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InventorsFlasinski, Stanislaw, Wilkinson, Jack Q., Fincher, Karen L.
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Primary Examiner(s)Guzo, David
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Assistant Examiner(s)MARVICH, MARIA
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Application NumberUS09/737,626Publication NumberTime in Patent Office1,089 DaysField of Search435/69.1, 435/69.7, 435/69.8, 435/70.1, 435/172.3, 435/183, 435/71.1, 435/320.1, 435/410, 435/413, 800/205, 800/255, 800/250, 800/300, 800/DIG.2756, 800/278, 935/35, 935/48, 935/67, 935/64, 935/29, 935/74, 935/9, 935/14, 935/30, 47/58, 536/23.1, 536/23.2, 536/23.6, 536/24.1US Class Current800/300CPC Class CodesA01N 57/20 containing acyclic or cyclo...C12N 15/8216 Methods for controlling, re...C12N 15/8221 Transit peptidesC12N 15/8222 Developmentally regulated e...C12N 15/8223 Vegetative tissue-specific ...C12N 15/823 Reproductive tissue-specifi...C12N 15/8261 with agronomic (input) trai...C12N 15/8275 GlyphosateC12N 15/8286 for insect resistanceC12N 2710/00043 viral genome or elements th...C12N 7/00 Viruses; Bacteriophages; Co...C12N 9/1092 3-Phosphoshikimate 1-carbox...Y02A 40/146 Genetically Modified [GMO] ...
