Low lipoxygenase 1 barley

• US 6,660,915 B2
• Filed: 12/29/2000
• Issued: 12/09/2003
• Est. Priority Date: 12/29/2000
• Status: Expired due to Term

• Current Assignee: Brasseries Kronenbourg Societe En Commandite Par Actions, Brasseries Kronenbourg (Carlsberg A/S), Carlsberg Research Laboratory, Heineken Technical Services B.V.
• Original Assignee: Brasseries Kronenbourg (Carlsberg A/S), Carlsberg Research Laboratory, Heineken Technical Services B.V.
• Inventors: Skadhauge, Birgitte, Doderer, Albert, Schmitt, Natalie, van Mechelen, Johannes Reinier, Heistek, Jolanda Carolina, Douma, Anna Christina, Cameron-Mills, Verena, Bech, Lene Moelskov
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): Kallis, Russell
• Application Number:
  US09/751,687
• Publication Number:
  US 20030167544A1
• Time in Patent Office: 1,075 Days
• Field of Search: 800/298, 800/278, 800/320, 435/185, 435/7, 435/6, 435/183, 426/392, 426/7, 426/11, 426/64
• US Class Current: 800/320
• CPC Class Codes: C12C 1/18 Preparation of malt extract or of special kinds of malt, e.g. caramel, black malt malt products for use as foodstuffs A23L
• CPC Class Codes: C12C 12/00 Processes specially adapted for making special kinds of beer
• CPC Class Codes: C12N 15/8242 with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
• CPC Class Codes: C12N 15/8243 involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Abstract

Barley plants having reduced lipoxygenase-1 enzyme activity are provided, for example, barley plants expressing mutant LOX-1 protein. The barley plants of the invention are useful in the production of plant products such as malt and brewed beverages, particularly beer, having increased stability and reduced T2N potential.

Claims

1. A barley plant or portion thereof, comprising a mutated LOX-1 protein having the amino acid sequence of SEQ ID NO:

12, wherein Xaa is an acidic, basic, or polar amino acid, the plant or portion characterized by a reduction or absence of LOX-1 activity as compared with a non-mutated control.

2. The barley plant or portion of claim 1, wherein Xaa is glutamic acid or aspartic acid.

3. The barley plant or portion of claim 2, wherein Xaa is aspartic acid.

4. Grain or plant progeny produced from the barley plant or plant portion and comprising the mutated LOX-1 protein of claim 1.

5. A plant product produced from the barley plant or plant portion and comprising the mutated LOX-1 protein of claim 1.

6. A method for producing a beverage having stable organoleptic qualities, comprising brewing of the plant or plant portion of claim 1 comprising the mutated LOX-1 protein, wherein the beverage presents stable organoleptic qualities that remain stable over a measured period of time or at elevated storage temperatures as compared with a control beverage.

7. A method for producing beer comprising brewing beer from the barley plant or plant portion of claim 1 comprising the mutated LOX-1 protein.

8. A method for producing a beer having stabilized organoleptic properties, comprising brewing beer from the barley plant or plant portion of claim 1 comprising the mutated LOX-1 protein, wherein said brewed beer has reduced levels of trans-2-nonenal over a measured period of time or under conditions of elevated storage temperature, as compared with a control beer brewed with a non-mutated barley.

9. The plant product of claim 5, wherein the product is malt.

10. A method for producing a beverage having stable organoleptic qualities, comprising processing the plant or plant portion of claim 1 comprising the mutated LOX-1 protein into a beverage wherein the beverage presents stable organoleptic qualities that remain stable over a measured period of time or at elevated storage temperatures as compared with a control beverage.

11. Malt produced by malting the grain of claim 4.