Nucleic acids encoding hedgehog proteins
First Claim
Patent Images
1. An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence at least 80 percent identical to a protein sequence selected from at least one of SEQ ID No:
- 8, SEQ ID No;
10, SEQ ID No;
11, SEQ ID No;
12, SEQ ID No;
13, SEQ ID No;
14 or an N-terminal fragment of at least 150 contiguous amino acid residues of said protein sequence, which amino acid sequence is not identical to SEQ ID No;
34, and wherein said polypeptide binds to a naturally occurring patched protein.
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Abstract
The present invention concerns the discovery that proteins encoded by a family of vertebrate genes, termed here hedgehog-related genes, comprise morphogenic signals produced by tissue patterning centers, and are involved in the formation of ordered spatial arrangements of differentiated tissues in vertebrates. The present invention makes available compositions and methods that can be utilized, for example to generate and/or maintain an array of different vertebrate tissue both in vitro and in vivo.
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Citations
35 Claims
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1. An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence at least 80 percent identical to a protein sequence selected from at least one of SEQ ID No:
- 8, SEQ ID No;
10, SEQ ID No;
11, SEQ ID No;
12, SEQ ID No;
13, SEQ ID No;
14 or an N-terminal fragment of at least 150 contiguous amino acid residues of said protein sequence, which amino acid sequence is not identical to SEQ ID No;
34, and wherein said polypeptide binds to a naturally occurring patched protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- 8, SEQ ID No;
-
20. An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence which nucleic acid hybridizes under stringent conditions, including a wash step of 0.2×
- SSC at 65°
C., to a nucleic acid sequence selected from at least one of SEQ ID No;
1, SEQ ID No;
3, SEQ ID No;
4, SEQ ID No;
5, SEQ ID No;
6 or SEQ ID No;
7, which polypeptide binds to a naturally occurring patched protein. - View Dependent Claims (21, 22, 23, 24, 25, 26)
- SSC at 65°
-
27. An isolated nucleic acid encoding a polypeptide, which polypeptide comprises an amino acid sequence represented by the formula A-B wherein,
A represents an amino acid sequence identical to residues 1-168 of SEQ ID No: - 40; and
B represents at least one amino acid residue of the amino acid sequence designated by residues 169-221 of SEQ ID No;
40;
wherein A and B together represent a contiguous polypeptide sequence identical to the corresponding sequence represented in SEQ ID No;
40, and said polypeptide binds to a naturally occurring patched protein.- View Dependent Claims (28, 29, 30, 31)
- 40; and
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32. An isolated nucleic acid encoding a polypeptide, which polypeptide comprises an amino acid sequence represented by the formula A-B wherein,
A represents an amino acid sequence identical to residues 24-193 of SEQ ID No: - 13; and
B represents at least one amino acid residue of the amino acid sequence designated by residues 194-250 of SEQ ID No;
13;
wherein A and B together represent a contiguous polypeptide sequence at least 80 percent identical to SEQ ID No;
13, and said polypeptide binds to a naturally occurring patched protein.
- 13; and
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33. An isolated nucleic acid encoding a polypeptide, which polypeptide comprises an amino acid sequence represented by the formula A-B wherein,
A represents an amino acid sequence identical to residues 25-193 of SEQ ID No: - 11; and
B represents at least one amino acid residue of the amino acid sequence designated by residues 194-250 of SEQ ID No;
11;
wherein A and B together represent a contiguous polypeptide sequence at least 80 percent identical to SEQ ID No;
11, and said polypeptide binds to a naturally occurring patched protein.
- 11; and
-
34. An isolated nucleic acid encoding a polypeptide, which polypeptide comprises an amino acid sequence represented by the formula A-B wherein,
A represents an amino acid sequence identical to residues 28-197 of SEQ ID No: - 10; and
B represents at least one amino acid residue of the amino acid sequence designated by residues 198-250 of SEQ ID No;
10;
wherein A and B together represent a contiguous polypeptide sequence at least 80 percent identical to SEQ ID No;
10, and said polypeptide binds a naturally occurring patched protein.
- 10; and
-
35. An isolated nucleic acid encoding a polypeptide, which polypeptide comprises an amino acid sequence represented by the general formula A-B wherein,
A represents an amino acid sequence identical to residues 1-98 of SEQ ID No: - 14; and
B represents at least one amino acid residues of the amino acid sequence designated by residues 99-150 of SEQ ID No;
14;
wherein A and B together represent a contiguous polypeptide sequence at least 80 percent identical to SEQ ID No;
14, and said polypeptide binds a naturally occurring patched protein.
- 14; and
Specification