Method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly) peptide complex
First Claim
1. A method for identifying a combination of nucleic acid sequences encoding two members of a multimeric polypeptide complex with a predetermined property, said combination being (i) located on separate nucleic acid molecules and (ii) contained in a combinatorial library of phage particles displaying a multitude of multimeric polypeptide complexes, said method comprising the steps (a) screening or selecting for polyphage particles that contain said combination, wherein said two members of the multimeric polypeptide complex interact to form an immunoglobulin, and wherein said predetermined property is the ability to bind to a target;
- and (b) identifying said combination of nucleic acid sequences.
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Accused Products
Abstract
The present invention relates to methods for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex by screening for polyphage particles. Furthermore, the invention relates to products and uses thereof for the identification of nucleic acid sequences in accordance with the present invention.
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Citations
36 Claims
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1. A method for identifying a combination of nucleic acid sequences encoding two members of a multimeric polypeptide complex with a predetermined property, said combination being (i) located on separate nucleic acid molecules and (ii) contained in a combinatorial library of phage particles displaying a multitude of multimeric polypeptide complexes, said method comprising the steps (a) screening or selecting for polyphage particles that contain said combination, wherein said two members of the multimeric polypeptide complex interact to form an immunoglobulin, and wherein said predetermined property is the ability to bind to a target;
- and (b) identifying said combination of nucleic acid sequences.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
Specification