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cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells

  • US 6,667,176 B1
  • Filed: 10/10/2000
  • Issued: 12/23/2003
  • Est. Priority Date: 01/11/2000
  • Status: Expired due to Term
First Claim
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1. A method of obtaining an mRNA preparation or a cDNA library from undifferentiated primate pluripotent stem (pPS) cells or their differentiated progeny, comprising:

  • a) providing undifferentiated pPS cells cultured in a suitable nutrient medium on an extracellular matrix but essentially free of feeder cells, b) optionally permitting the pPS cells to differentiate, and c) isolating mRNA from the undifferentiated or differentiated cells.

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