Devices and methods for detecting analytes using electrosensor having capture reagent
First Claim
Patent Images
1. A device for detecting an analyte in a liquid sample, which device comprises:
- a) a solid support;
b) an electrosensor immobilized on said solid support, said electrosensor comprises a working electrode and another electrode used as auxiliary and/or reference electrode;
c) a capture reagent immobilized on said working electrode, said capture reagent binds to an analyte, if said analyte is present in a liquid sample; and
d) conductive leads for connecting said electrodes to a readout device for electrochemical measurement, wherein the device further comprising a sample application area that is separate, but in fluid communication with the electrosensor via a wicking member having an open mesh structure that does not comprise an immobilized capture reagent, and wherein the wicking member having an open mesh structure provides for two dimensional transport of fluids from the application area to the electrosensor and faster flow rate, lower background noise and higher signal to noise ratio compared to a wicking member not having an open mesh structure.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to devices comprising electrosensors containing capture reagents, their preparation thereof, and their use for detecting, preferably, quantitative measurement, of analyte in a liquid sample. In particular, the invention relates to an enzyme electrosensor, e.g., electroimmunosensor, device for electrochemical detection and preferably, real-time measurement, which is suitable for use at point-of-care settings by unskilled personnel.
-
Citations
36 Claims
-
1. A device for detecting an analyte in a liquid sample, which device comprises:
-
a) a solid support;
b) an electrosensor immobilized on said solid support, said electrosensor comprises a working electrode and another electrode used as auxiliary and/or reference electrode;
c) a capture reagent immobilized on said working electrode, said capture reagent binds to an analyte, if said analyte is present in a liquid sample; and
d) conductive leads for connecting said electrodes to a readout device for electrochemical measurement, wherein the device further comprising a sample application area that is separate, but in fluid communication with the electrosensor via a wicking member having an open mesh structure that does not comprise an immobilized capture reagent, and wherein the wicking member having an open mesh structure provides for two dimensional transport of fluids from the application area to the electrosensor and faster flow rate, lower background noise and higher signal to noise ratio compared to a wicking member not having an open mesh structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 31, 34, 35, 36)
a) the device of claim 1; and
b) an effective amount of an electron transfer mediator and a substrate.
-
-
34. The device of claim 1, wherein the wicking member comprises a nylon net.
-
35. The device of claim 34, wherein the nylon net has a mesh opening from 11 μ
- m to 100 μ
m.
- m to 100 μ
-
36. The device of claim 34, wherein the nylon net has a mesh opening from 80 μ
- m to 100 μ
m.
- m to 100 μ
-
30. A device for detecting an analyte in a liquid sample, which device comprises:
-
a) a base sensor strip having a working electrode, a reference electrode, and an auxiliary electrode coated on a plastic substrate, whereon a capture reagent is immobilized on the working electrode, said sensor strip having conductive leads for attaching the electrodes to a readout device for electrochemical measurement;
b) a cover casing having a liquid sample application aperture and a detection aperture;
c) an application zone for receiving a fluid containing an analyte from the application aperture, said application zone, in dry unused form, containing a labeled detection reagent specifically binds to said analyte, if said analyte is present said fluid, wherein said labeled reagent is released into mobile form when in contact with the liquid sample;
d) a detection zone in fluid communication with the electrodes in the presence of a liquid sample received from the application aperture;
e) a wicking member having an open mesh structure that does not comprise an immobilized capture reagent and that carries the liquid sample from the application zone to the detection zone by capillary action, wherein the wicking member having an open mesh structure provides for two dimensional transport of fluids from the application area to the electrosensor and faster flow rate, lower background noise and higher signal to noise ratio compared to a wicking member not having an open mesh structure and wherein said analyte is sandwiched between the detection reagent and the capture reagent immobilized on the electrode surface; and
f) an absorbent sink placed in partial contact with the wicking member at the end of the flow path to absorb any excess fluid from the detection zone.
-
- 32. A device for detecting an analyte in a liquid sample, which device comprises a sample application area that is in fluid communication with an electrosensor via a wicking member, wherein the wicking member has an open mesh structure that does not comprises an immobilized capture reagent that binds to an analyte, and wherein the wicking member having an open mesh structure provides for two dimensional transport of fluids from the application area to the electrosensor and faster flow rate, lower background noise and higher signal to noise ratio compared to a wicking member not having an open mesh structure.
Specification