Microfluidic devices and methods for performing temperature mediated reactions
First Claim
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1. A microfluidic device, comprising:
- a body structure, comprising;
a reaction channel with a first electrical resistance, wherein the reaction channel has primers but no polymerase disposed therein;
first and second electrical access channels having a second electrical resistance, wherein said first electrical resistance is greater than said second electrical resistance, said electrical access channels fluidly connected to said reaction channel; and
a source of polymerase enzyme fluidly connected to said reaction channel at a first intersection.
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Abstract
Microfluidic devices with improved channel and reservoir configurations are provided. More specifically, efficient microfluidic devices for the performance of temperature mediated reactions are provided. These reactions can be performed in an ease of use and efficient manner so as to enable high through put of multiple samples. Methods for performing temperature mediated reactions using the microfluidic devices with improved channel and reservoir configurations have also been provided.
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15 Claims
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1. A microfluidic device, comprising:
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a body structure, comprising;
a reaction channel with a first electrical resistance, wherein the reaction channel has primers but no polymerase disposed therein;
first and second electrical access channels having a second electrical resistance, wherein said first electrical resistance is greater than said second electrical resistance, said electrical access channels fluidly connected to said reaction channel; and
a source of polymerase enzyme fluidly connected to said reaction channel at a first intersection. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification