Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof

• US 6,673,597 B2
• Filed: 02/28/2001
• Issued: 01/06/2004
• Est. Priority Date: 06/02/2000
• Status: Active Grant

• Current Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Original Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Inventors: Goodwin, Thomas J., Wolf, David A.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/798,854
• Publication Number:
  US 20020009797A1
• Time in Patent Office: 1,042 Days
• Field of Search: 435/173.1, 435/173.8, 435/298.2, 435/299.1
• US Class Current: 435/298.2
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Claims

1. A system for growing biological cells, comprising:

a rotating cell culture vessel;

a cell culture medium contained within said rotating cell culture vessel;

an electrode; and

an electrical circuit connected to said electrode for passing a time varying electrical current through said electrode for generating a time varying electromagnetic field within said cell culture medium.

2. The system of claim 1, wherein said time varying electrical current is in the form of an alternating current.

3. The system of claim 1, wherein said time varying electrical current is in the form of a square wave.

4. The system of claim 1, wherein said electrode is adjacent to and spaced from said rotational cell culture vessel.

5. The system of claim 1, wherein said electrode is positioned within said rotational cell culture vessel.

6. The system of claim 1, wherein said electrical current is from about 1 mA to about 1,000 mA.

7. The system of claim 6, wherein said time varying electrical current is about 1 milliamperes to about 6 milliamperes.

8. The system of claim 1, wherein said time varying electromagnetic field induces a cellular response at gene level.

9. The system of claim 8, wherein said cellular response is cellular control of growth and differentiation at gene level.

10. The system of claim 9, wherein said cellular control of growth and differentiation is to suppress or enhance growth regulatory functions at gene level.

11. The system of claim 10, wherein said gene is associated with embryogenesis.

12. The system of claim 1, wherein said electrode is made of a material selected from the group consisting of titanium, zirconium, platinum and stainless steel.

13. The system of claim 1, wherein said electrode comprises an antenna for emitting said time varying electromagnetic field within said cell culture medium.

14. The system of claim 13, wherein said antenna is positioned adjacent said rotating cell culture vessel, said antenna comprising means for inducing an electromagnetic field within said medium within said rotating cell culture vessel.

15. The system for growing biological cells of claim 1, wherein the frequency of said time varying electrical current is approximately 10 Herz.

16. A system for growing biological cells in a two-dimensional array, wherein said cells exhibit accelerated growth, comprising:

a cell culture medium;

a container;

an electrode positioned within said container;

said biological cells, wherein said biological cells contained in said cell culture medium are bubbled onto said electrode; and

an electrical circuit connected to said electrode for passing a time varying electrical current through said electrode for generating a time varying electromagnetic field within said cell culture medium.

17. The system of claim 16, wherein said electrode is positioned adjacent said container.

18. The system of claim 16, wherein said electrode is positioned within said container.

19. The system of claim 16, wherein said biological cells comprise neural cells.

20. The system of claim 16, further comprising:

a slide placed on said electrode wherein said biological cells contained in said cell culture medium are bubbled thereon.

21. The system of claim 16, wherein said time varying electrical current is in the form of an alternating current.

22. The system of claim 16, wherein said time varying electrical current is in the form of a square wave.

23. The system of claim 16, wherein said electrical current is from about 1 millamperes to about 1,000 1 millamperes.

24. The system of claim 16, wherein said electrical current is from about 1 millamperes to about 6 millamperes.

25. The system of claim 16, wherein said electrical is made of a material selected from the group consisting of titanium, zirconium, platinum and stainless steel.