Human tumor-associated gene

• US 6,673,914 B1
• Filed: 01/21/1999
• Issued: 01/06/2004
• Est. Priority Date: 01/22/1998
• Status: Expired due to Term

• Current Assignee: John Wayne Cancer Institute
• Original Assignee: John Wayne Cancer Institute
• Inventors: Hoon, David S. B.
• Primary Examiner(s): HELMS, LARRY RONALD
• Application Number:
  US09/234,685
• Time in Patent Office: 1,811 Days
• Field of Search: 536/23.1, 536/23.5, 536/24.5, 536/24.1, 536/24.37, 530/350, 435/320.1, 435/325, 435/243, 435/252.3, 435/252.33, 435/254.1, 435/358
• US Class Current: 536/23.5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4748 Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
• CPC Class Codes: G01N 33/5017 for testing neoplastic activity
• CPC Class Codes: G01N 33/57407 Specifically defined cancers
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites

Abstract

The present invention describes a novel tumor marker antigen encoded by a gene designated as HOJ-1 (SEQ ID NO:1). In specific embodiment, the nucleic acid sequences disclosed herein are for used in the diagnosis and prognosis of cancer. Also provided are related protein and antibody compositions and various methods of use thereof, including methods for cancer diagnosis and treatment.

Claims

1. An isolated DNA segment that encodes a HOJ-1 polypeptide comprising the amino acid sequence of SEQ ID NO:

2.

2. The isolated DNA segment of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

1.

3. The isolated DNA segment of claim 1, wherein the DNA segment is positioned under the control of a promoter.

4. The isolated DNA segment of claim 1, further defined as a recombinant vector.

15. The isolated DNA segment of claim 1, further comprising an origin of replication.

16. A bacterial vector or a plasmid vector that comprises the DNA segment of claim 15.

17. The isolated DNA segment of claim 1, further comprising a polyadenylation signal operably linked to the segment encoding HOJ-1.

18. The isolated DNA segment of claim 15, wherein said DNA segment is a viral vector selected from the group consisting of retrovirus, adenovirus, herpesvirus, vaccinia virus, pox-virus and adeno-associated virus.

5. An isolated recombinant host cell comprising an isolated DNA segment that comprises a DNA segment that encodes a HOJ-1 protein comprising the amino acid sequence of SEQ ID NO:

2.

6. The recombinant host cell of claim 5, further defined as a prokaryotic host cell.

7. The recombinant host cell of claim 5, further defined as a eukaryotic host cell.

8. The recombinant host cell of claim 5, wherein the DNA segment is introduced into the cell by means of a recombinant vector.

9. The recombinant host cell of claim 5, wherein the DNA segment has the sequence of SEQ ID NO:

1.

10. An isolated nucleic acid segment or 14 to no more than 100 contiguous nucleotides from bases 1-868 of SEQ ID NO:

1, or the fill complement thereof, wherein the complement is a contiguous segment of 14 to no more than 100 nucleotides.

11. The isolated nucleic acid segment of claim 10, wherein said isolated nucleic acid segment is 20 to no more than 100 contiguous nucleotides from bases 1-868 of SEQ ID NO:

1, or the full complement thereof, wherein the complement is a contiguous segment of 20 to no more than 100 nucleotides.

12. The isolated nucleic acid segment of claim 10, wherein said isolated nucleic acid segment is 25 to no more than 100 contiguous nucleotides from bases 1-868 of SEQ ID NO:

1, or the full complement thereof, wherein the complement is a contiguous segment of 25 to no more than 100 nucleotides.

13. The isolated nucleic acid segment of claim 10, further defined as a DNA segment.

14. The isolated nucleic acid segment of claim 10, further defined as a RNA segment.

19. A composition of matter comprising a DNA segment that encodes a HOJ-1 polypeptide comprising the amino acid sequence of SEQ ID NO:

2 packaged in a virus particle.

20. A composition of matter comprising a DNA segment that encodes a HOJ-1 polypeptide comprising the amino acid sequence of SEQ ID NO:

2 packaged in a liposome.